ArticleMicrobiome2020
Entomopathogenic nematode-associated microbiota: from monoxenic paradigm to pathobiome.
Article in Microbiome, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 97 citations in OpenAlex.
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- Escherichia coli Nissle 1917 Occupies Previously Undocumented Host Niches in the Insect-Parasitic Nematode Steinernema hermaphroditum.Environmental microbiology reports · 2026Article
- Substitutions in the cardiac glycoside binding site of the NaJournal of invertebrate pathology · 2026Article
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- Genomes of the entomopathogenic nematode Steinernema hermaphroditum and its associated bacteria.Genetics · 2025Article
- ParaRef: a decontaminated reference database for parasite detection in ancient and modern metagenomic datasets.Genome biology · 2025Article
- Pleiotropic role of PAX cyclolipopeptides in theApplied and environmental microbiology · 2025Article
- Mitigation of chytridiomycosis onMicrobiology spectrum · 2025Article
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- Master of Puppets: How Microbiota Drive the Nematoda Ecology and Evolution?Ecology and evolution · 2025Review
- Epistasis between NabioRxiv : the preprint server for biology · 2025Article
- The evaluation of shotgun sequencing and rpoB metabarcoding for taxonomic profiling of bacterial communities.BMC microbiology · 2025Article
- Microbiome dynamics associated with the infection of grey field slugs by the biocontrol nematodeFrontiers in microbiology · 2025Article
- Genome sequences ofMicrobiology resource announcements · 2024Article
- ExploringMolecules (Basel, Switzerland) · 2024Review
- Systematics and phylogeny of the entomopathogenic nematobacterial complexes Steinernema-Xenorhabdus and Heterorhabditis-Photorhabdus.Zoological letters · 2024Review
- OxyR is required for oxidative stress resistance of the entomopathogenic bacteriumMicrobiology (Reading, England) · 2024Article
- Review
- Early transcriptomic responses of rice leaves to herbivory by Spodoptera frugiperda.Scientific reports · 2024Article
- The evolution of entomopathogeny in nematodes.Ecology and evolution · 2024Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe holistic view of bacterial symbiosis, incorporating both host and microbial environment, constitutes a major conceptual shift in studies deciphering host-microbe interactions. Interactions between Steinernema entomopathogenic nematodes and their bacterial symbionts, Xenorhabdus, have long been considered monoxenic two partner associations responsible for the killing of the insects and therefore widely used in insect pest biocontrol. We investigated this "monoxenic paradigm" by profiling the microbiota of infective juveniles (IJs), the soil-dwelling form responsible for transmitting Steinernema-Xenorhabdus between insect hosts in the parasitic lifecycle.
resultsMultigenic metabarcoding (16S and rpoB markers) showed that the bacterial community associated with laboratory-reared IJs from Steinernema carpocapsae, S. feltiae, S. glaseri and S. weiseri species consisted of several Proteobacteria. The association with Xenorhabdus was never monoxenic. We showed that the laboratory-reared IJs of S. carpocapsae bore a bacterial community composed of the core symbiont (Xenorhabdus nematophila) together with a frequently associated microbiota (FAM) consisting of about a dozen of Proteobacteria (Pseudomonas, Stenotrophomonas, Alcaligenes, Achromobacter, Pseudochrobactrum, Ochrobactrum, Brevundimonas, Deftia, etc.). We validated this set of bacteria by metabarcoding analysis on freshly sampled IJs from natural conditions. We isolated diverse bacterial taxa, validating the profile of the Steinernema FAM. We explored the functions of the FAM members potentially involved in the parasitic lifecycle of Steinernema. Two species, Pseudomonas protegens and P. chlororaphis, displayed entomopathogenic properties suggestive of a role in Steinernema virulence and membership of the Steinernema pathobiome.
conclusionsOur study validates a shift from monoxenic paradigm to pathobiome view in the case of the Steinernema ecology. The microbial communities of low complexity associated with EPNs will permit future microbiota manipulation experiments to decipher overall microbiota functioning in the infectious process triggered by EPN in insects and, more generally, in EPN ecology.
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