ArticleMicrobial cell factories2020
Optimized expression and enhanced production of alkaline protease by genetically modified Bacillus licheniformis 2709.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 60 citations in OpenAlex.
- System reconstruction ofSynthetic and systems biotechnology · 2027Article
- Efficient Expression of Small Molecule Bioactive Peptides in Bacillus licheniformis.Applied biochemistry and biotechnology · 2026Article
- Recombinant thermotolerant alkaline lipase from Lysinibacillus fusiformis for detergent and hard (Ras) cheese applications: cloning, expression, molecular docking, and characterization.Microbial cell factories · 2026Article
- Precision hydrolysis: tailored yeast processing enzymes for yeast-based products.Applied microbiology and biotechnology · 2026Review
- Exploration of Multi-Source Lignocellulose-Degrading Microbial Resources and Bioaugmentation Strategies: Implications for Rumen Efficiency.Animals : an open access journal from MDPI · 2025Review
- Effect of Fold-Promoting Mutation and Signal Peptide Screening on Recombinant Glucan 1,4-Alpha-maltohydrolase Secretion in Pichia pastoris.Applied biochemistry and biotechnology · 2025Article
- Enhancing the Thermostability ofMolecules (Basel, Switzerland) · 2025Article
- Inactivation ofInternational journal of molecular sciences · 2024Article
- Lignocellulolytic Enzymes Production by Four Wild Filamentous Fungi for Olive Stones Valorization: Comparing Three Fermentation Regimens.Journal of microbiology and biotechnology · 2024Article
- Utilizing chitooligosaccharides from shrimp waste biodegradation via recombinant chitinase A: a promising approach for emulsifying hydrocarbon and bioremediation.Microbial cell factories · 2024Article
- Advances in recombinant protease production: current state and perspectives.World journal of microbiology & biotechnology · 2024Review
- Secretory expression of amylosucrase in Bacillus licheniformis through twin-arginine translocation pathway.Journal of industrial microbiology & biotechnology · 2024Article
- Creating Single-Cell Protein-ProducingScientifica · 2024Article
- Biotechnological and food synthetic biology potential of platform strain:Synthetic and systems biotechnology · 2023Review
- Enhancement of alkaline protease production in recombinant Bacillus licheniformis by response surface methodology.Bioresources and bioprocessing · 2023Article
- Isolation and identification of protease-producingFrontiers in bioengineering and biotechnology · 2023Article
- Multiple Modular Engineering ofFrontiers in bioengineering and biotechnology · 2022Article
- Overexpression ofFrontiers in microbiology · 2022Article
- Article
- Microbial proteases: ubiquitous enzymes with innumerable uses.3 Biotech · 2021Review
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6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundBacillus licheniformis 2709 is extensively applied as a host for the high-level production of heterologous proteins, but Bacillus cells often possess unfavorable wild-type properties, such as production of viscous materials and foam during fermentation, which seriously influenced the application in industrial fermentation. How to develop it from a soil bacterium to a super-secreting cell factory harboring less undomesticated properties always plays vital role in industrial production. Besides, the optimal expression pattern of the inducible enzymes like alkaline protease has not been optimized by comparing the transcriptional efficiency of different plasmids and genomic integration sites in B. licheniformis.
resultBacillus licheniformis 2709 was genetically modified by disrupting the native lchAC genes related to foaming and the eps cluster encoding the extracellular mucopolysaccharide via a markerless genome-editing method. We further optimized the expression of the alkaline protease gene (aprE) by screening the most efficient expression system among different modular plasmids and genomic loci. The results indicated that genomic expression of aprE was superior to plasmid expression and finally the transcriptional level of aprE greatly increased 1.67-fold through host optimization and chromosomal integration in the vicinity of the origin of replication, while the enzyme activity significantly improved 62.19% compared with the wild-type alkaline protease-producing strain B. licheniformis.
conclusionWe successfully engineered an AprE high-yielding strain free of undesirable properties and its fermentation traits could be applied to bulk-production by host genetic modification and expression optimization. In summary, host optimization is an enabling technology for improving enzyme production by eliminating the harmful traits of the host and optimizing expression patterns. We believe that these strategies can be applied to improve heterologous protein expression in other Bacillus species.
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