ArticleMicrobial cell factories2020
Expression regulation of multiple key genes to improve L-threonine in Escherichia coli.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 35 citations in OpenAlex.
- Efficient production of glycine from glucose via a two-stage biosynthetic strategy.Microbial cell factories · 2026Article
- Whey as an Environmental Issue and Its Possible Solutions: Its Utilization as Culture Medium to Produce L-Threonine ThroughBiochemistry research international · 2026Review
- Engineering Escherichia coli for Efficient Production of L-Tryptophan.Applied biochemistry and biotechnology · 2025Article
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- Engineering of increased L-Threonine production in bacteria by combinatorial cloning and machine learning.Metabolic engineering communications · 2023Article
- l-Threonine production from whey and fish hydrolysate byHeliyon · 2023Article
- Dynamic and balanced regulation of theFrontiers in bioengineering and biotechnology · 2023Article
- Blue Light Signaling Regulates Escherichia coli W1688 Biofilm Formation and l-Threonine Production.Microbiology spectrum · 2022Article
- Metabolic engineering of Escherichia coli for quinolinic acid production by assembling L-aspartate oxidase and quinolinate synthase as an enzyme complex.Metabolic engineering · 2021Article
- Improving L-threonine production in Escherichia coli by elimination of transporters ProP and ProVWX.Microbial cell factories · 2021Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundEscherichia coli is an important strain for L-threonine production. Genetic switch is a ubiquitous regulatory tool for gene expression in prokaryotic cells. To sense and regulate intracellular or extracellular chemicals, bacteria evolve a variety of transcription factors. The key enzymes required for L-threonine biosynthesis in E. coli are encoded by the thr operon. The thr operon could coordinate expression of these genes when L-threonine is in short supply in the cell.
resultsThe thrL leader regulatory elements were applied to regulate the expression of genes iclR, arcA, cpxR, gadE, fadR and pykF, while the threonine-activating promoters P
conclusionThe genetic engineering through the expression regulation of key genes is a better strategy than simple deletion of these genes to improve L-threonine production in E. coli. This strategy has little effect on the intracellular metabolism in the early stage of the growth but could increase L-threonine biosynthesis in the late stage.
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