ArticleMalaria journal2020
Member species of the Anopheles gambiae complex can be misidentified as Anopheles leesoni.
Article in Malaria journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- A multiplex assay to detect mosquito species, bloodmeal host source and Plasmodium in malaria vectors using Nanopore amplicon sequencing.Medical and veterinary entomology · 2026Article
- Mosquito vector composition and biting behavior of malaria vectors in forest versus humid savanna eco-epidemiological settings of Cameroon.Malaria journal · 2026Article
- Mosquito Feeding Preference, Infectivity Rates, and Knockdown Resistance Within the Wild Population ofTropical medicine and infectious disease · 2025Article
- Assessing Anopheles species collection techniques in a low malaria transmission area: implications for vector surveillance and control.Malaria journal · 2025Article
- MALDI-TOF MS for malaria vector surveillance: A cost-comparison analysis using a decision-tree approach.PloS one · 2025Article
- Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin.BMC infectious diseases · 2024Article
- Anopheles arabiensis continues to be the primary vector of Plasmodium falciparum after decades of malaria control in southwestern Ethiopia.Malaria journal · 2024Article
- Analysis of geometric morphometrics and molecular phylogeny for Anopheles species in the Republic of Korea.Scientific reports · 2023Article
- Rapid and non-destructive identification of Anopheles gambiae and Anopheles arabiensis mosquito species using Raman spectroscopy via machine learning classification models.Malaria journal · 2023Article
- Anopheles mosquito diversity, entomological indicators of malaria transmission and challenges of morphological identification in southwestern Ethiopia.Tropical medicine and health · 2023Article
- High vector diversity and malaria transmission dynamics in five sentinel sites in Cameroon.Malaria journal · 2023Article
- Myzomyia and Pyretophorus series of Anopheles mosquitoes acting as probable vectors of the goat malaria parasite Plasmodium caprae in Thailand.Scientific reports · 2023Article
- No evidence of lymphatic filariasis transmission in Bamako urban setting after three mass drug administration rounds.Parasitology research · 2022Article
- Myzorhynchus series of Anopheles mosquitoes as potential vectors of Plasmodium bubalis in Thailand.Scientific reports · 2022Article
- Article
- The dynamic gut microbiota of zoophilic members of the Anopheles gambiae complex (Diptera: Culicidae).Scientific reports · 2022Article
- An Ecologically Framed Comparison of The Potential for Zoonotic Transmission of Non-Human and Human-Infecting Species of Malaria Parasite.The Yale journal of biology and medicine · 2021Review
- Extensive new Anopheles cryptic species involved in human malaria transmission in western Kenya.Scientific reports · 2020Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundAccurate Anopheles species identification is key for effective malaria vector control. Identification primarily depends on morphological analysis of field samples as well as molecular species-specific identifications. During an intra-laboratory assessment (proficiency testing) of the Anopheles funestus group multiplex PCR assay, it was noted that Anopheles arabiensis can be misidentified as Anopheles leesoni, a zoophilic member of the An. funestus group. The aim of this project was, therefore, to ascertain whether other members of the Anopheles gambiae complex can also be misidentified as An. leesoni when using the standard An. funestus multiplex PCR.
methodsThe An. funestus multiplex PCR was used to amplify DNA from An. gambiae complex specimens. These included specimens from the laboratory colonies and field samples from the Democratic Republic of Congo. Amplified DNA from these specimens, using the universal (UV) and An. leesoni species-specific primers (LEES), were sequence analysed. Additionally, An. leesoni DNA was processed through the diagnostic An. gambiae multiplex PCR to determine if this species can be misidentified as a member of the An. gambiae complex.
resultsLaboratory-colonized as well as field-collected samples of An. arabiensis, An. gambiae, Anopheles merus, Anopheles quadriannulatus, Anopheles coluzzii as well as Anopheles moucheti produced an amplicon of similar size to that of An. leesoni when using an An. funestus multiplex PCR. Sequence analysis confirmed that the UV and LEES primers amplify a segment of the ITS2 region of members of the An. gambiae complex and An. moucheti. The reverse was not true, i.e. the An. gambiae multiplex PCR does not amplify DNA from An. leesoni.
conclusionThis investigation shows that An. arabiensis, An. gambiae, An. merus, An. quadriannulatus, An. coluzzii and An. moucheti can be misidentified as An. leesoni when using An. funestus multiplex PCR. This shows the importance of identifying specimens using standard morphological dichotomous keys as far as possible prior to the use of appropriate PCR-based identification methods. Should there be doubt concerning field-collected specimens molecularly identified as An. leesoni, the An. gambiae multiplex PCR and sequencing of the internal transcribed spacer 2 (ITS2) can be used to eliminate false identifications.
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