Evidence map›Paper›PMID 32093646›Full record

ArticleBMC microbiology2020

The Oxidoreductase DsbA1 negatively influences 2,4-diacetylphloroglucinol biosynthesis by interfering the function of Gcd in Pseudomonas fluorescens 2P24.

Bo Zhang, Hui Zhao, Xiaogang Wu, Li-Qun Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.6field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. New Insights intoAntibiotics (Basel, Switzerland) · 2024
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Bo ZhangCollege of Agriculture, Guangxi University, Nanning, 530004, China.
Hui ZhaoCollege of Plant Protection, China Agricultural University, Beijing, 100193, China.
Xiaogang WuCollege of Agriculture, Guangxi University, Nanning, 530004, China. 4146308505@outlook.com.
Li-Qun ZhangCollege of Plant Protection, China Agricultural University, Beijing, 100193, China. zhanglq@cau.edu.cn.
China Agricultural University · CNGuangxi University · CN

Funding

National Major Science and Technology Projects of China (CN) AA17204041National Natural Science Foundation of China 31760533National Natural Science Foundation of China 31872020the National Key Research and Development Program of China 2017YFD02011083the Natural Science Foundation of Guangxi 2017GXNSFAA198341
6 · The paper itself

Abstract

backgroundThe polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG), produced by Pseudomonas fluorescens 2P24, is positively regulated by the GacS-GacA two-component system.

resultsHere we reported on the characterization of DsbA1 (disulfide oxidoreductase) as novel regulator of biocontrol activity in P. fluorescens. Our data showed that mutation of dsbA1 caused the accumulation of 2,4-DAPG in a GacA-independent manner. Further analysis indicated that DsbA1 interacts with membrane-bound glucose dehydrogenase Gcd, which positively regulates the production of 2,4-DAPG. Mutation of cysteine (C)-235, C275, and C578 of Gcd, significantly reduced the interaction with DsbA1, enhanced the activity of Gcd and increased 2,4-DAPG production.

conclusionsOur results suggest that DsbA1 regulates the 2,4-DAPG concentration via fine-tuning the function of Gcd in P. fluorescens 2P24.

Indexed as

Bacterial ProteinsBinding SitesCysteineGene Expression Regulation, BacterialGlucose DehydrogenasesMutationOxidoreductasesPhloroglucinolProtein BindingPseudomonas fluorescens2,4-diacetylphloroglucinolBacterial ProteinsCysteineGlucose DehydrogenasesOxidoreductasesPhloroglucinol2,4-DAPGDisulfide bondGlucose dehydrogenase GcdOxidoreductase DsbA1Pseudomonas fluorescens

Identifiers

PMID32093646
PMCPMC7041245
OpenAlexW3011006801

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.