ArticlePloS one2020
Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- Advances in the Molecular Regulatory Mechanisms of Testicular Development and Spermatogenesis in Yaks.Animals : an open access journal from MDPI · 2026Review
- Metabolomics Reveals Abnormal Citrate Cycle and Phenylalanine Metabolism in Testes from Infertile Hybrid Dzo.Animals : an open access journal from MDPI · 2025Article
- Molecular characterization and expression ofVeterinary and animal science · 2025Review
- Comparative Analysis of Testicular Transcriptional and Translational Landscapes in Yak and Cattle-Yak: Implications for Hybrid Male Sterility.Biomolecules · 2025Article
- Sex-biased gene expression precedes sexual dimorphism in the agonadal annelid Platynereis dumerilii.Development (Cambridge, England) · 2025Article
- Genetic insights into non-obstructive azoospermia: Implications for diagnosis and TESE outcomes.Journal of assisted reproduction and genetics · 2025Article
- Dissecting genomes of multiple yak populations: unveiling ancestry and high-altitude adaptation through whole-genome resequencing analysis.BMC genomics · 2025Article
- Comparative RNA-Seq analysis of differentially expressed genes in the sertoli cells of yak and cattle-yak.BMC veterinary research · 2025Article
- NLife (Basel, Switzerland) · 2024Article
- Sex-biased gene expression precedes sexual dimorphism in the agonadal annelidbioRxiv : the preprint server for biology · 2024Article
- mRNA-Seq of testis and liver tissues reveals a testis-specific gene and alternative splicing associated with hybrid male sterility in dzo.Journal of animal science · 2024Article
- Genome-wide scan for runs of homozygosity in South American Camelids.BMC genomics · 2023Article
- Review
- Single-cell transcriptome analysis and in vitro differentiation of testicular cells reveal novel insights into male sterility of the interspecific hybrid cattle-yak.BMC genomics · 2023Article
- The Biological Characteristics and Differential Expression Patterns ofAnimals : an open access journal from MDPI · 2023Article
- Analysis of Chromatin Openness in Testicle Tissue of Yak and Cattle-Yak.International journal of molecular sciences · 2022Article
- First detection and complete genome analysis of porcine circovirus-like virus P1 and porcine circovirus-2 in yak in China.Veterinary medicine and science · 2022Article
- F1 Male Sterility in Cattle-Yak Examined through Changes in Testis Tissue and Transcriptome Profiles.Animals : an open access journal from MDPI · 2022Article
- Within- and between-Breed Selection Signatures in the Original and Improved Valachian Sheep.Animals : an open access journal from MDPI · 2022Article
- ProAKAP4 Semen Concentrations as a Valuable Marker Protein of Post-Thawed Semen Quality and Bull Fertility: A Retrospective Study.Veterinary sciences · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCattleyak are the hybrid offspring between cattle and yak and combine yak hardiness with cattle productivity. Much attempt has been made to examine the mechanisms of male sterility caused by spermatogenic arrest, but yet there is no research systematically and precisely elucidated testis gene expression profiling between cattleyak and yak.
methodsTo explore the higher resolution comparative transcriptome map between the testes of yak and cattleyak, and further analyze the mRNA expression dynamics of spermatogenic arrest in cattleyak. We characterized the comparative transcriptome profile from the testes of yak and cattleyak using high-throughput sequencing. Then we used quantitative analysis to validate several differentially expressed genes (DEGs) in testicular tissue and spermatogenic cells.
resultsTestis transcriptome profiling identified 6477 DEGs (2919 upregulated and 3558 downregulated) between cattleyak and yak. Further analysis revealed that the marker genes and apoptosis regulatory genes for undifferentiated spermatogonia were upregulated, while the genes for differentiation maintenance were downregulated in cattleyak. A majority of DEGs associated with mitotic checkpoint, and cell cycle progression were downregulated in cattleyak during spermatogonial mitosis. Furthermore, almost all DEGs related to synaptonemal complex assembly, and meiotic progression presented no sign of expression in cattleyak. Even worse, dozens of genes involved in acrosome formation, and flagellar development were dominantly downregulated in cattleyak.
conclusionDEGs indicated that spermatogenic arrest of cattleyak may originate from the differentiation stage of spermatogonial stem cells and be aggravated during spermatogonial mitosis and spermatocyte meiosis, which contributes to the scarcely presented sperms in cattleyak.
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