Evidence map›Paper›PMID 32090156›Full record

ArticleBone reports2020

Characterization of a reproducible model of fracture healing in mice using an open femoral osteotomy.

C D Collier, B S Hausman, S H Zulqadar, E S Din, J M Anderson, O Akkus, E M Greenfield

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bone reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 29 citations in OpenAlex.

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  16. Methodology, selection, and integration of fracture healing assessments in mice.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

C D CollierDepartment of Orthopaedics, University Hospitals Cleveland Medical Center, Case Western Reserve University, Cleveland, OH 44106, USA.
B S HausmanDepartment of Orthopaedics, University Hospitals Cleveland Medical Center, Case Western Reserve University, Cleveland, OH 44106, USA.
S H ZulqadarDepartment of Orthopaedics, University Hospitals Cleveland Medical Center, Case Western Reserve University, Cleveland, OH 44106, USA.
E S DinDepartment of Orthopaedics, University Hospitals Cleveland Medical Center, Case Western Reserve University, Cleveland, OH 44106, USA.
J M AndersonDepartment of Pathology, Case Western Reserve University, Cleveland, OH 44106, USA.
O AkkusDepartment of Orthopaedics, University Hospitals Cleveland Medical Center, Case Western Reserve University, Cleveland, OH 44106, USA.
E M GreenfieldDepartment of Orthopaedics, University Hospitals Cleveland Medical Center, Case Western Reserve University, Cleveland, OH 44106, USA.
University Hospitals of Cleveland · USCase Western Reserve University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe classic fracture model, described by Bonnarens and Einhorn in 1984, enlists a blunt guillotine to generate a closed fracture in a pre-stabilized rodent femur. However, in less experienced hands, this technique yields considerable variability in fracture pattern and requires highly-specialized equipment. This study describes a reproducible and low-cost model of mouse fracture healing using an open femoral osteotomy.

methodsFemur fractures were produced in skeletally mature male and female mice using an open femoral osteotomy after intramedullary stabilization. Mice were recovered for up to 28 days prior to analysis with microradiographs, histomorphometry, a novel μCT methodology, and biomechanical torsion testing at weekly intervals.

resultsEight mice were excluded due to complications (8/193, 4.1%), including unacceptable fracture pattern (2/193, 1.0%). Microradiographs showed progression of the fracture site to mineralized callus by 14 days and remodelling 28 days after surgery. Histomorphometry from 14 to 28 days revealed decreased cartilage area and maintained bone area. μCT analysis demonstrated a reduction in mineral surface from 14 to 28 days, stable mineral volume, decreased strut number, and increased strut thickness. Torsion testing at 21 days showed that fractured femurs had 61% of the ultimate torque, 63% of the stiffness, and similar twist to failure when compared to unfractured contralateral femurs.

conclusionsThe fracture model described herein, an open femoral osteotomy, demonstrated healing comparable to that reported using closed techniques. This simple model could be used in future research with improved reliability and reduced costs compared to the current options.

Indexed as

Bone repairFracture healingMicro-CT fracture analysisMouse fracture modelOsteotomy

Identifiers

PMID32090156
PMCPMC7025178
OpenAlexW3004660282

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.