ArticleThe EMBO journal1988
Visualization of a filamentous nucleoskeleton with a 23 nm axial repeat.
Article in The EMBO journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- Long non-coding RNAs: roles in cellular stress responses and epigenetic mechanisms regulating chromatin.Nucleus (Austin, Tex.) · 2024Review
- Ubiquitin C-terminal hydrolase isozyme L1 is associated with shelterin complex at interstitial telomeric sites.Epigenetics & chromatin · 2017Article
- Organization of nuclear architecture during adipocyte differentiation.Nucleus (Austin, Tex.) · 2016Review
- Genome-wide mapping of matrix attachment regions in Drosophila melanogaster.BMC genomics · 2014Article
- A requiem to the nuclear matrix: from a controversial concept to 3D organization of the nucleus.Chromosoma · 2014Review
- Relationship between DNA replication and the nuclear matrix.Genes to cells : devoted to molecular & cellular mechanisms · 2013Review
- Dynamics of nuclear matrix proteome during embryonic development in Drosophila melanogaster.Journal of biosciences · 2011Article
- Nuclear scaffold attachment sites within ENCODE regions associate with actively transcribed genes.PloS one · 2011Article
- Inheriting nuclear organization: can nuclear lamins impart spatial memory during post-mitotic nuclear assembly?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Lamin B1 maintains the functional plasticity of nucleoli.Journal of cell science · 2009Article
- Silencing by nuclear matrix attachment distinguishes cell-type specificity: association with increased proliferation capacity.Nucleic acids research · 2009Article
- Maintenance of a functional higher order chromatin structure: The role of the nuclear matrix in normal and disease states.Gene therapy & molecular biology · 2009Article
- In-silico prediction and observations of nuclear matrix attachment.Cellular & molecular biology letters · 2006Article
- Nucleoskeleton of early bovine embryos and differentiated somatic cells: an ultrastructural and immunocytochemical comparison.Histochemistry and cell biology · 2004Article
- Amino-terminal polypeptides of vimentin are responsible for the changes in nuclear architecture associated with human immunodeficiency virus type 1 protease activity in tissue culture cells.Molecular biology of the cell · 2001Article
- Disruption of nuclear lamin organization blocks the elongation phase of DNA replication.The Journal of cell biology · 2000Article
- Intranuclear anchoring of repetitive DNA sequences: centromeres, telomeres, and ribosomal DNA.The Journal of cell biology · 1999Article
- Direct visualization of a protein nuclear architecture.Molecular biology of the cell · 1999Article
- The nuclear matrix prepared by amine modification.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Direct visualization of the human estrogen receptor alpha reveals a role for ligand in the nuclear distribution of the receptor.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whether nucleoskeletons seen after extracting cells are preparative artefacts is controversial. Using an extraction method that preserves vital nuclear functions, we have visualized part of a nucleoskeleton by electron microscopy of thick resinless sections. Cells encapsulated in agarose microbeads are lysed using Triton in a physiological buffer; the agarose coat prevents aggregation and protects fragile cell contents. These extracted cells are accessible to small molecules and transcribe and replicate at rates close to those in vivo. After electroeluting most chromatin after treatment with HaeIII, a skeleton is uncovered which ramifies throughout the nucleus. Individual filaments are approximately 10 nm wide with an axial repeat of 23 nm, characteristic of intermediate filaments.
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Registered trials
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