ArticleNucleic acids research2020
The fungal CCAAT-binding complex and HapX display highly variable but evolutionary conserved synergetic promoter-specific DNA recognition.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed.
- Iron starvation confers azole tolerance inmBio · 2026Article
- Siderophore production by the lichen fungus Xanthoria parietina supports its algal symbiont.Nature communications · 2026Article
- Genome-wide discovery and phenotyping of non-coding transcripts in A. fumigatus reveals lncRNAs with a role in antifungal drug sensitivity.Nature communications · 2026Article
- The iron-responsive transcription factor HapX drives iron starvation adaptation and virulence inCurrent research in microbial sciences · 2026Article
- Functional transitions of the Aspergillus fumigatus iron regulator HapX are governed by conserved domains cooperatively binding [2Fe-2S] clusters.Nucleic acids research · 2025Article
- Engineering of Global Transcriptional Regulators (GTRs) inJournal of fungi (Basel, Switzerland) · 2025Review
- Regulation of Ergosterol Biosynthesis in Pathogenic Fungi: Opportunities for Therapeutic Development.Microorganisms · 2025Review
- The transcriptional regulatorCurrent research in microbial sciences · 2025Article
- Article
- The Transcription Factors AcuK and AcuM Influence Siderophore Biosynthesis ofJournal of fungi (Basel, Switzerland) · 2024Article
- HapX-mediated H2B deub1 and SreA-mediated H2A.Z deposition coordinate in fungal iron resistance.Nucleic acids research · 2023Article
- Article
- The Regulatory Hub of Siderophore Biosynthesis in the Phytopathogenic FungusJournal of fungi (Basel, Switzerland) · 2023Article
- Structure of the HapX:CCAAT-binding protein complex with DNA: A piece of the puzzle revealed.Structure (London, England : 1993) · 2022Article
- Exploring a novel genomic safe-haven site in the human pathogenic mould Aspergillus fumigatus.Fungal genetics and biology : FG & B · 2022Article
- Synergistic Antifungal Effect of a Combination of Iron Deficiency and Calcium Supplementation.Microbiology spectrum · 2022Article
- Azole Resistance-Associated Regulatory Motifs within the Promoter ofMicrobiology spectrum · 2022Article
- Role of the Heme Activator Protein Complex in the Sexual Development of Cryptococcus neoformans.mSphere · 2022Article
- Article
- Article
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14 authors.
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No grant is acknowledged in the PubMed record.
Abstract
To sustain iron homeostasis, microorganisms have evolved fine-tuned mechanisms for uptake, storage and detoxification of the essential metal iron. In the human pathogen Aspergillus fumigatus, the fungal-specific bZIP-type transcription factor HapX coordinates adaption to both iron starvation and iron excess and is thereby crucial for virulence. Previous studies indicated that a HapX homodimer interacts with the CCAAT-binding complex (CBC) to cooperatively bind bipartite DNA motifs; however, the mode of HapX-DNA recognition had not been resolved. Here, combination of in vivo (genetics and ChIP-seq), in vitro (surface plasmon resonance) and phylogenetic analyses identified an astonishing plasticity of CBC:HapX:DNA interaction. DNA motifs recognized by the CBC:HapX protein complex comprise a bipartite DNA binding site 5'-CSAATN12RWT-3' and an additional 5'-TKAN-3' motif positioned 11-23 bp downstream of the CCAAT motif, i.e. occasionally overlapping the 3'-end of the bipartite binding site. Phylogenetic comparison taking advantage of 20 resolved Aspergillus species genomes revealed that DNA recognition by the CBC:HapX complex shows promoter-specific cross-species conservation rather than regulon-specific conservation. Moreover, we show that CBC:HapX interaction is absolutely required for all known functions of HapX. The plasticity of the CBC:HapX:DNA interaction permits fine tuning of CBC:HapX binding specificities that could support adaptation of pathogens to their host niches.
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