ArticleCell2020
A Metabolic Pathway for Activation of Dietary Glucosinolates by a Human Gut Symbiont.
Article in Cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
79 citing papers in PubMed, 1 synthesis or guideline pooled it, 144 citations in OpenAlex.
- Meta-analysis of the gut microbiota in predicting response to cancer immunotherapy in metastatic melanoma.JCI insight · 2020Pooled it
- Exogenous myrosinase from mustard seed increases bioavailability of sulforaphane from a glucoraphanin-rich broccoli seed extract in a randomized clinical study.Scientific reports · 2026Trial
- Effect of broccoli sprout extract and baseline gut microbiota on fasting blood glucose in prediabetes: a randomized, placebo-controlled trial.Nature microbiology · 2025Trial
- In vitro digestion modifies Lepidium meyenii extract, altering its major glucosinolates, lepidilines, β-carbolines, and gut bacterial growth.Journal of food and drug analysis · 2026Article
- How Gut Microbes Transform Dietary Phytonutrients: Enzymatic Pathways and Human Metabolite Exposure.Molecules (Basel, Switzerland) · 2026Review
- The Gut Microbiome and Diet Interact to Dictate Personalized Response to Broccoli Sprout Consumption.Food science & nutrition · 2026Article
- Sulforaphane-ProducingFood science & nutrition · 2026Article
- Mechanisms and Implications of Gut Microbiota-Derived Metabolites in the Regulation of Poultry Defensins.Animals : an open access journal from MDPI · 2026Review
- Bioresponsive pseudoGlucosinolates (psGSLs) Release Isothiocyanates (ITCs) in the Presence of Nitroreductases.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- The microbiome regulates host metabolic health and diseases through microbial enzymes.Nature reviews. Gastroenterology & hepatology · 2026Review
- ATML1-GIR1-TPL/TPR transcriptional repression module controls glucosinolates and giant cells inbioRxiv : the preprint server for biology · 2026Article
- Glucosinolate Derivatives: Emerging Anti-Inflammatory Agents.Pharmaceuticals (Basel, Switzerland) · 2026Review
- The Adaptive Laboratory Evolution Technology Enhances Sulforaphane Production of Lactobacillus plantarum CR12.Probiotics and antimicrobial proteins · 2026Article
- Gut microbiome metabolism of plant phenolic glycosides and its relationship with human health.Microbiome research reports · 2026Article
- Gut microbiota gate host exposure to cholinesterase inhibitors from dietary Solanums.bioRxiv : the preprint server for biology · 2025Article
- Carbohydrates and the oxidative branch of the pentose phosphate pathway modifyJournal of bacteriology · 2025Article
- Beyond the Tongue: Brassica Vegetables, Distributed Taste Receptors, and Their Role in Holistic Health.Integrative medicine (Encinitas, Calif.) · 2025Article
- Food as Medicine for Hypertension: Microbiota as Mediators.Hypertension (Dallas, Tex. : 1979) · 2025Review
- Bioavailability, Human Metabolism, and Dietary Interventions of Glucosinolates and Isothiocyanates: Critical Insights and Future Perspectives.Foods (Basel, Switzerland) · 2025Review
- Cruciferous Vegetables, Bioactive Metabolites, and Microbiome for Breast Cancer Prevention.Annual review of nutrition · 2025Review
19 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Consumption of glucosinolates, pro-drug-like metabolites abundant in Brassica vegetables, has been associated with decreased risk of certain cancers. Gut microbiota have the ability to metabolize glucosinolates, generating chemopreventive isothiocyanates. Here, we identify a genetic and biochemical basis for activation of glucosinolates to isothiocyanates by Bacteroides thetaiotaomicron, a prominent gut commensal species. Using a genome-wide transposon insertion screen, we identified an operon required for glucosinolate metabolism in B. thetaiotaomicron. Expression of BT2159-BT2156 in a non-metabolizing relative, Bacteroides fragilis, resulted in gain of glucosinolate metabolism. We show that isothiocyanate formation requires the action of BT2158 and either BT2156 or BT2157 in vitro. Monocolonization of mice with mutant BtΔ2157 showed reduced isothiocyanate production in the gastrointestinal tract. These data provide insight into the mechanisms by which a common gut bacterium processes an important dietary nutrient.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.