ArticleScience advances2020
Attoliter protein nanogels from droplet nanofluidics for intracellular delivery.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Biosensing With Nanofluidics.Exploration (Beijing, China) · 2026Review
- Microgels for Cell Delivery in Tissue Engineering and Regenerative Medicine.Nano-micro letters · 2024Review
- Preparation and biomedical applications of artificial cells.Materials today. Bio · 2023Review
- Microfluidics-Enabled Nanovesicle Delivers CD47/PD-L1 Antibodies to Enhance Antitumor Immunity and Reduce Immunotoxicity in Lung Adenocarcinoma.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Article
- Microfluidic Methods for Generation of Submicron Droplets: A Review.Micromachines · 2023Review
- Experimental study on the electrohydrodynamic deformation of droplets in a combined DC electric field and shear flow field.Fundamental research · 2023Article
- Selenium Silk Nanostructured Films with Antifungal and Antibacterial Activity.ACS applied materials & interfaces · 2023Article
- Gas-assisted microfluidic step-emulsification for generating micron- and submicron-sized droplets.Microsystems & nanoengineering · 2023Article
- Attomole-Level Multiplexed Detection of Neurochemicals in Picoliter Droplets by On-Chip Nanoelectrospray Ionization Coupled to Mass Spectrometry.Analytical chemistry · 2022Article
- Hydrodynamics of Droplet Sorting in Asymmetric Acute Junctions.Micromachines · 2022Article
- Adsorption free energy predicts amyloid protein nucleation rates.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Hydrogels as functional components in artificial cell systems.Nature reviews. Chemistry · 2022Review
- Microfluidics Fabrication of Micrometer-Sized Hydrogels with Precisely Controlled Geometries for Biomedical Applications.Advanced healthcare materials · 2022Review
- Volumetric Scalability of Microfluidic and Semi-Batch Silk Nanoprecipitation Methods.Molecules (Basel, Switzerland) · 2022Article
- Mixing and flow-induced nanoprecipitation for morphology control of silk fibroin self-assembly.RSC advances · 2022Article
- Protein nanoparticles directed cancer imaging and therapy.Nano convergence · 2022Review
- Droplet Microfluidics for Tumor Drug-Related Studies and Programmable Artificial Cells.Global challenges (Hoboken, NJ) · 2021Review
- From Protein Building Blocks to Functional Materials.ACS nano · 2021Review
- Synthesis of Nanogels: Current Trends and Future Outlook.Gels (Basel, Switzerland) · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Microscale hydrogels consisting of macromolecular networks in aqueous continuous phases have received increasing attention because of their potential use in tissue engineering, cell encapsulation and for the storage and release of cargo molecules. However, for applications targeting intracellular delivery, their micrometer-scale size is unsuitable for effective cellular uptake. Nanoscale analogs of such materials are thus required for this key area. Here, we describe a microfluidics/nanofluidics-based strategy for generating monodisperse nanosized water-in-oil emulsions with controllable sizes ranging from 2500 ± 110 nm down to 51 ± 6 nm. We demonstrate that these nanoemulsions can act as templates to form protein nanogels stabilized by supramolecular fibrils from three different proteins. We further show that these nanoparticles have the ability to penetrate mammalian cell membranes and deliver intracellular cargo. Due to their biocompatibility and lack of toxicity, natural protein-based nanoparticles present advantageous characteristics as vehicles for cargo molecules in the context of pharmaceutical and biomedical applications.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.