ArticleFacts, views & vision in ObGyn2019
Thaw, biopsy and refreeze strategy for PGT-A on previously cryopreserved embryos.
Article in Facts, views & vision in ObGyn, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Embryonic and neonatal outcomes following double vitrification/thawing: a systematic review and meta-analysis.BMC pregnancy and childbirth · 2025Pooled it
- Impacts of double biopsy and double vitrification on the clinical outcomes following euploid blastocyst transfer: a systematic review and meta-analysis.Human reproduction (Oxford, England) · 2024Pooled it
- Impact of repeated cryopreservation on embryo development and chromosomal ploidy.Archives of gynecology and obstetrics · 2026Article
- Double vitrification-warming cycles coupled with one blastocyst biopsy do not affect the chance of live birth after an euploid blastocyst transfer: a propensity score matching study.Journal of translational medicine · 2025Article
- Effect of re-biopsy and re-vitrification on clinical outcomes in preimplantation genetic testing for aneuploidy.Journal of assisted reproduction and genetics · 2025Article
- Accumulation of Cleavage-Stage Embryos by Vitrification may Compromise Embryonic Developmental Potential in Preimplantation Genetic Testing.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Live birth rate following a euploid blastocyst transfer is not affected by double vitrification and warming at cleavage or blastocyst stage.Journal of assisted reproduction and genetics · 2022Article
- Effect of the Re-Vitrification of Embryos at Different Stages on Embryonic Developmental Potential.Frontiers in endocrinology · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPreimplantation genetic testing for aneuploidy (PGT-A) with Next Generation Sequencing technology is a low-cost and powerful technology for the analysis of embryo quality. However, PGT-A requires freezing of embryos, suggesting that previously cryopreserved embryos cannot be tested. Here, we test whether use of the vitrification technique permits the refreezing of embryos, thus permitting PGT-A testing of cryopreserved embryos.
methodsThe results are a retrospective analysis of cases performed at Create fertility between 2016 and 2017. Results obtained after traditional PGT-A are compared with results after the thaw biopsy and refreeze (TBR) procedure. A total of 220 patients were treated with PGT-A and 54 patients with the TBR procedure.
resultsMaternal ages were not significantly different between the groups. The proportion of PGT-A normal embryos was not significantly different between the two groups. A clinical pregnancy rate of 61.5% was achieved with the PGT-A group and 52.4% with the TBR group. These results were not statistically significant. The efficiency of the thaw, biopsy and refreeze technique was not significantly different to that of fresh cycles for rates of survival, results obtained and aneuploidy incidence. Clinical pregnancy rates are not significantly different after the biopsy of fresh and previously cryopreserved embryos.
conclusionThe data shows that the TBR procedure has an equivalent success rate to that of classical PGT-A procedures.
Indexed as
Identifiers
32082528PMC7020945What OpenQuestion holds
Registered trials
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