ArticleNature communications2020
Serial protein crystallography in an electron microscope.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
- Characterization of energy filtering slit widths for MicroED data collection.Journal of structural biology: X · 2026Article
- Tracking the redox reaction of the iron enzyme ribonucleotide reductase using continuous SerialED and SFX.Structure (London, England : 1993) · 2026Article
- Automated serial electron diffraction: implementation inJournal of applied crystallography · 2026Article
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
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- Article
- Single-cell structural biology with intracellular electron crystallography.Nature communications · 2026Article
- Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals.Acta crystallographica. Section D, Structural biology · 2026Article
- Limiting the effects of radiation damage in MicroED through dose selection during data processing.Acta crystallographica. Section D, Structural biology · 2025Article
- Automated and real-time structure solution using 3D electron diffraction.Journal of applied crystallography · 2025Article
- Serial Chemical Crystallography for Autonomous Quantitative Phase Analysis in an Electron Microscope.Small methods · 2025Article
- Deciphering Evolution, Function, and Observation of Crystallization-Driven Self-Assembly.Chemical reviews · 2025Review
- Standard Sample Preparation for Serial Femtosecond Crystallography.Biomolecules · 2025Review
- Reuniting crystallography with real space: Ab initio structure elucidation with 4D-STEM.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Machine learning for the classification of serial electron diffraction patterns: synthetic data.Acta crystallographica. Section A, Foundations and advances · 2025Article
- Combining MicroED and native mass spectrometry for structural discovery of enzyme-small molecule complexes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- On the use of beam precession for serial electron crystallography.Journal of applied crystallography · 2025Article
- 3D Electron Diffraction on Nanoparticles: Minimal Size and Associated Dynamical Effects.ACS nano · 2025Article
- Vertical DNA Nanostructure Arrays: Facilitating Functionalization on Macro-Scale Surfaces.ACS nano · 2025Article
- Review
12 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serial X-ray crystallography at free-electron lasers allows to solve biomolecular structures from sub-micron-sized crystals. However, beam time at these facilities is scarce, and involved sample delivery techniques are required. On the other hand, rotation electron diffraction (MicroED) has shown great potential as an alternative means for protein nano-crystallography. Here, we present a method for serial electron diffraction of protein nanocrystals combining the benefits of both approaches. In a scanning transmission electron microscope, crystals randomly dispersed on a sample grid are automatically mapped, and a diffraction pattern at fixed orientation is recorded from each at a high acquisition rate. Dose fractionation ensures minimal radiation damage effects. We demonstrate the method by solving the structure of granulovirus occlusion bodies and lysozyme to resolutions of 1.55 Å and 1.80 Å, respectively. Our method promises to provide rapid structure determination for many classes of materials with minimal sample consumption, using readily available instrumentation.
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