Evidence map›Paper›PMID 32081583›Full record

ArticleUltrasound in medicine & biology2020

Effects of Non-invasive, Targeted, Neuronal Lesions on Seizures in a Mouse Model of Temporal Lobe Epilepsy.

Yanrong Zhang, Haiyan Zhou, Haibo Qu, Chengde Liao, Hong Jiang, Siqin Huang, Sara Natasha Ghobadi, Arsenii Telichko, Ningrui Li, Frezghi G Habte and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ultrasound in medicine & biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Review
  6. Article
  7. Acoustic Holograms for Bilateral Blood-Brain Barrier Opening in a Mouse Model.IEEE transactions on bio-medical engineering · 2022
    Article
  8. Review
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yanrong ZhangNeuroradiology Section, Department of Radiology, School of Medicine, Stanford University, California, USA.
Haiyan ZhouNeuroradiology Section, Department of Radiology, School of Medicine, Stanford University, California, USA; The Acupuncture and Tuina School/Third Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
Haibo QuNeuroradiology Section, Department of Radiology, School of Medicine, Stanford University, California, USA; Department of Medical Imaging, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
Chengde LiaoNeuroradiology Section, Department of Radiology, School of Medicine, Stanford University, California, USA; Department of Radiology, Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming, Yunnan, China.
Hong JiangNeuroradiology Section, Department of Radiology, School of Medicine, Stanford University, California, USA; Department of Neurology, Peking University of People's Hospital, Beijing, China.
Siqin HuangNeuroradiology Section, Department of Radiology, School of Medicine, Stanford University, California, USA; Traditional Chinese Medicine College, Chongqing Medical University, Chongqing, China.
Sara Natasha GhobadiNeuroradiology Section, Department of Radiology, School of Medicine, Stanford University, California, USA.
Arsenii TelichkoDepartment of Radiology, Stanford University, Stanford, California, USA.
Ningrui LiDepartment of Radiology, Stanford University, Stanford, California, USA.
Frezghi G HabteMolecular Imaging Program at Stanford, Department of Radiology, Stanford, California, USA.
Tim DoyleMolecular Imaging Program at Stanford, Department of Radiology, Stanford, California, USA.
James P WoznakDepartment of Neuroscience, University of Virginia, Charlottesville, Virginia, USA.
Edward H BertramDepartment of Neurology, University of Virginia, Charlottesville, Virginia, USA.
Kevin S LeeDepartment of Neuroscience, University of Virginia, Charlottesville, Virginia, USA; Department of Neurosurgery and Center for Brain Immunology and Glia, School of Medicine, University of Virginia, Charlottesville, Virginia, USA. Electronic address: ksl3h@virginia.edu.
Max WintermarkNeuroradiology Section, Department of Radiology, School of Medicine, Stanford University, California, USA. Electronic address: max.wintermark@gmail.com.

Funding

MR-Guided Focused Ultrasound Combined with Immunotherapy to Treat Malignant Brain TumorsR01CA217953 · NCI · STANFORD UNIVERSITY · PI HEIT, JEREMY J · 2017 to 2021
$3.1M
Precise, non-invasive, axon-sparing surgery for the treatment of drug resistant epilepsyR01NS102194 · NINDS · UNIVERSITY OF VIRGINIA · PI LEE, KEVIN SCOTT · 2018 to 2022
$2.1M
NCI NIH HHS R01 CA217953NINDS NIH HHS R01 NS102194
6 · The paper itself

Abstract

Surgery to treat drug-resistant epilepsy can be quite effective but remains substantially underutilized. A pilot study was undertaken to test the feasibility of using a non-invasive, non-ablative, approach to produce focal neuronal loss to treat seizures in a rodent model of temporal lobe epilepsy. In this study, spontaneous, recurrent seizures were established in a mouse model of pilocarpine-induced status epilepticus. After post-status epilepticus stabilization, baseline behavioral seizures were monitored for 30 d. Non-invasive opening of the blood-brain barrier targeting the hippocampus was then produced by using magnetic resonance-guided, low-intensity focused ultrasound, through which a neurotoxin (quinolinic acid) administered intraperitoneally gained access to the brain parenchyma to produce focal neuronal loss. Behavioral seizures were then monitored for 30 d after this procedure, and brains were subsequently prepared for histologic analysis of the sites of neuronal loss. The average frequency of behavioral seizures in all animals (n = 11) was reduced by 21.2%. Histologic analyses along the longitudinal axis of the hippocampus revealed that most of the animals (n = 8) exhibited neuronal loss located primarily in the intermediate aspect of the hippocampus, while sparing the septal aspect. Two other animals with damage to the intermediate hippocampus also exhibited prominent bilateral damage to the septal aspect of the hippocampus. A final animal had negligible neuronal loss overall. Notably, the site of neuronal loss along the longitudinal axis of the hippocampus influenced seizure outcomes. Animals that did not have bilateral damage to the septal hippocampus displayed a mean decrease in seizure frequency of 27.7%, while those with bilateral damage to the septal hippocampus actually increased seizure frequency by 18.7%. The animal without neuronal loss exhibited an increase in seizure frequency of 19.6%. The findings indicate an overall decrease in seizure frequency in treated animals. And, the site of neuronal loss along the longitudinal axis of the hippocampus appears to play a key role in reducing seizure activity. These pilot data are promising, and they encourage additional and more comprehensive studies examining the effects of targeted, non-invasive, neuronal lesions for the treatment of epilepsy.

Indexed as

Ultrasonic Surgical ProceduresAnimalsBlood-Brain BarrierDisease Models, AnimalEpilepsy, Temporal LobeFeasibility StudiesMagnetic Resonance ImagingMaleMiceMicrobubblesNeuronsPilocarpinePilot ProjectsPilocarpineEpilepsy surgeryFocused ultrasoundMagnetic resonance-guidedNeuronal lossNon-invasive

Identifiers

PMID32081583
PMCPMC8120598

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.