ArticleMetabolites2020
Addressing Glutathione Redox Status in Clinical Samples by Two-Step Alkylation with N-ethylmaleimide Isotopologues.
Article in Metabolites, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Cysteine/GSH-Responsive Lipid Switch Development for Liposome Triggered Cargo Release.Bioconjugate chemistry · 2026Article
- Single-atom Co-N-C nanozyme with enhanced oxidase-like activity for smartphone-assisted colorimetric detection of sulfite.Mikrochimica acta · 2026Article
- Elucidation of Sulforaphane-Mediated Effects on the Cellular Human Metabolome Using Metabolic Profiling.Molecular nutrition & food research · 2026Article
- LC-MS Evaluation of the Redox Trypanothione Balance inAntioxidants (Basel, Switzerland) · 2025Article
- Increased antioxidative defense and reduced advanced glycation end-product formation by metabolic adaptation in non-small-cell-lung-cancer patients.Nature communications · 2025Article
- Effect of N-Acetylcysteine in Mitochondrial Function, Redox Signaling, and Sirtuin 3 Levels in the Heart During Cardiorenal Syndrome Type 4 Development.Antioxidants (Basel, Switzerland) · 2025Article
- Article
- Oxidative Stress in Fetuses and Newborns.Antioxidants (Basel, Switzerland) · 2024Article
- Analytical Methods for Assessing Thiol Antioxidants in Biological Fluids: A Review.Molecules (Basel, Switzerland) · 2024Review
- Comparison of muscle metabolomics between two Chinese horse breeds.Frontiers in veterinary science · 2023Article
- Direct Derivatization in Dried Blood Spots for Oxidized and Reduced Glutathione Quantification in Newborns.Antioxidants (Basel, Switzerland) · 2022Article
- Let-7 underlies metformin-induced inhibition of hepatic glucose production.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Evidence on the Heroin-Mediated Impairment of the Oxidative Status of Erythrocytes.Journal of toxicology · 2022Article
- Glutathione: A Samsonian life-sustaining small molecule that protects against oxidative stress, ageing and damaging inflammation.Frontiers in nutrition · 2022Review
- Extensive Thiol Profiling for Assessment of Intracellular Redox Status in Cultured Cells by HPLC-MS/MS.Antioxidants (Basel, Switzerland) · 2021Article
- Extending the Scope ofAnalytical chemistry · 2021Article
- Comparative Analysis of Phenolic Composition of Six Commercially Available Chamomile (International journal of molecular sciences · 2021Article
- Blood Plasma Quality Control by Plasma Glutathione Status.Antioxidants (Basel, Switzerland) · 2021Article
- Glutathione and oxidized nicotinamide adenine dinucleotide (NADSaudi medical journal · 2021Article
- Susceptibility of the patients infected with Sars-Cov2 to oxidative stress and possible interplay with severity of the disease.Free radical biology & medicine · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Determination of the ratio of reduced to oxidized glutathione is of profound clinical interest in assessing the oxidative status of tissues and body fluids. However, this ratio is not yet a routine clinical parameter due to the analytically challenging interconversion of reduced (free) glutathione to oxidized (bound) glutathione. We aimed to facilitate this ratio determination in order to aid its incorporation as a routine clinical parameter. To this end, we developed a simple derivatization route that yields different isotopologues of N-ethylmaleimide alkylated glutathione from reduced and oxidized glutathione (after its chemical reduction) for mass spectrometric analysis. A third isotopologue can be used as isotopic standard for simultaneous absolute quantification. As all isotopologues have similar chromatographic properties, matrix effects arising from different sample origins can only impact method sensitivity but not quantification accuracy. Robustness, simplified data analysis, cost effectiveness by one common standard, and highly improved mass spectrometric sensitivity by conversion of oxidized glutathione to an alkylated glutathione isotopologue are the main advantages of our approach. We present a method fully optimized for blood, plasma, serum, cell, and tissue samples. In addition, we propose production of N-ethylmaleimide customized blood collection tubes to even further facilitate the analysis in a clinical setting.
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