Evidence map›Paper›PMID 32078767›Full record

ArticleACS chemical neuroscience2020

Photodynamic Modification of Native HCN Channels Expressed in Thalamocortical Neurons.

Fusheng Wei, Qiang Wang, Jizhong Han, Priyodarshan Goswamee, Ankush Gupta, Adam Rory McQuiston, Qinglian Liu, Lei Zhou

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS chemical neuroscience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Fusheng WeiDepartment of Physiology and Biophysics, Department of Anatomy and Neurobiology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23284, United States.
Qiang WangDepartment of Physiology and Biophysics, Department of Anatomy and Neurobiology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23284, United States.
Jizhong HanDepartment of Physiology and Biophysics, Department of Anatomy and Neurobiology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23284, United States.
Priyodarshan GoswameeDepartment of Physiology and Biophysics, Department of Anatomy and Neurobiology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23284, United States.
Ankush GuptaDepartment of Physiology and Biophysics, Department of Anatomy and Neurobiology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23284, United States.
Adam Rory McQuistonDepartment of Physiology and Biophysics, Department of Anatomy and Neurobiology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23284, United States.
Qinglian LiuDepartment of Physiology and Biophysics, Department of Anatomy and Neurobiology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23284, United States.
Lei ZhouDepartment of Physiology and Biophysics, Department of Anatomy and Neurobiology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23284, United States.ORCID 0000-0002-3838-1836
Virginia Commonwealth University · USNanchang University · CN

Funding

Structural and Functional Studies of Hsp70 Molecular ChaperonesR01GM098592 · NIGMS · VIRGINIA COMMONWEALTH UNIVERSITY · PI Qinglian Liu · 2013 to 2026
$4.3M
Cells, synapses, and networks contributing to synchrony in the CNSR01MH107507 · NIMH · VIRGINIA COMMONWEALTH UNIVERSITY · PI MCQUISTON, ADAM RORY · 2016 to 2020
$1.9M
Molecular biophysics of cAMP regulation in HCN channelsR01GM109193 · NIGMS · VIRGINIA COMMONWEALTH UNIVERSITY · PI LIU, QINGLIAN, ZHOU, LEI · 2014 to 2017
$1.4M
Effect of pathogenic tau on entorhinal cortical inputs to dentate gyrus interneuronsR21AG055073 · NIA · VIRGINIA COMMONWEALTH UNIVERSITY · PI MCQUISTON, ADAM RORY · 2017 to 2018
$426k
NIA NIH HHS R21 AG055073NIGMS NIH HHS R01 GM098592NIGMS NIH HHS R01 GM109193NIMH NIH HHS R01 MH107507
6 · The paper itself

Abstract

The photodynamic process requires three elements: light, oxygen, and photosensitizer, and involves the formation of singlet oxygen, the molecular oxygen in excited electronic states. Previously, we reported that heterologously expressed hyperpolarization-activated cAMP-gated (HCN) channels in excised membrane patches are sensitive to photodynamic modification (PDM). Here we extend this study to native HCN channels expressed in thalamocortical (TC) neurons in the ventrobasal (VB) complex of the thalamus and dopaminergic neurons (DA) of the ventral tegmental area (VTA). To do this, we introduced the photosensitizer FITC-cAMP into TCs or DAs of rodent brain slices via a whole-cell patch-clamp recording pipette. After illumination with blue light pulses, we observed an increase in the voltage-insensitive, instantaneous

Indexed as

Cerebral CortexHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsNeuronsAnimalsCyclic Nucleotide-Gated Cation ChannelsMembrane PotentialsMiceMice, Inbred C57BLPatch-Clamp TechniquesThalamusCyclic Nucleotide-Gated Cation ChannelsHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsHCN channelIh currentPhotodynamic modificationphotosensitizerthalamocortical neuronventral tegmental area

Identifiers

PMID32078767
PMCPMC7222614
OpenAlexW3006734912

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.