ArticlePLoS genetics2020
Planarian EGF repeat-containing genes megf6 and hemicentin are required to restrict the stem cell compartment.
Article in PLoS genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Injury-induced Neuregulin-EGFR signaling from muscle mobilizes stem cells for whole-body regeneration in acoels.Developmental biology · 2025Article
- Knockdown of Atg1 Impairs Brain Regeneration and Downregulates ECM-Related Genes in the Planarian Dugesia japonica.Molecular neurobiology · 2025Article
- HMCN1 variants aggravate epidermolysis bullosa simplex phenotype.The Journal of experimental medicine · 2025Article
- Article
- Extracellular Vesicles Derived From Regenerating Tissue Promote Stem Cell Proliferation in the PlanarianJournal of extracellular biology · 2025Article
- Single-cell RNA sequencing of the holothurian regenerating intestine reveals the pluripotency of the coelomic epithelium.bioRxiv : the preprint server for biology · 2024Article
- Extracellular vesicles promote proliferation in an animal model of regeneration.bioRxiv : the preprint server for biology · 2024Article
- Hemicentin-1 is an essential extracellular matrix component during tooth root formation by promoting mesenchymal cells differentiation.Frontiers in cell and developmental biology · 2024Article
- Vertebrate extracellular matrix protein hemicentin-1 interacts physically and genetically with basement membrane protein nidogen-2.Matrix biology : journal of the International Society for Matrix Biology · 2022Article
- Intestine-enriched apolipoprotein b orthologs are required for stem cell progeny differentiation and regeneration in planarians.Nature communications · 2022Article
- Positional Information and Stem Cells Combine to Result in Planarian Regeneration.Cold Spring Harbor perspectives in biology · 2022Review
- Articulating the "stem cell niche" paradigm through the lens of non-model aquatic invertebrates.BMC biology · 2022Review
- Decoding Stem Cells: An Overview on Planarian Stem Cell Heterogeneity and Lineage Progression.Biomolecules · 2021Review
- Hemicentin-1 is an essential extracellular matrix component of the dermal-epidermal and myotendinous junctions.Scientific reports · 2021Article
- Set1 Targets Genes with Essential Identity and Tumor-Suppressing Functions in Planarian Stem Cells.Genes · 2021Article
- RNA-Seq reveals divergent gene expression between larvae with contrasting trophic modes in the poecilogonous polychaete Boccardia wellingtonensis.Scientific reports · 2021Article
- Collagen IV differentially regulates planarian stem cell potency and lineage progression.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.Molecular & cellular proteomics : MCP · 2021Article
- A Rare Variant ofFrontiers in neurology · 2021Article
- Innovations and Advances in Schistosome Stem Cell Research.Frontiers in immunology · 2021Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The extracellular matrix (ECM) is important for maintaining the boundaries between tissues. This role is particularly critical in the stem cell niche, as pre-neoplastic or cancerous stem cells must pass these boundaries in order to invade into the surrounding tissue. Here, we examine the role of the ECM as a regulator of the stem cell compartment in the planarian Schmidtea mediterranea, a highly regenerative, long-lived organism with a large population of adult stem cells. We identify two EGF repeat-containing genes, megf6 and hemicentin, with identical knockdown phenotypes. We find that megf6 and hemicentin are needed to maintain the structure of the basal lamina, and in the absence of either gene, pluripotent stem cells migrate ectopically outside of their compartment and hyper-proliferate, causing lesions in the body wall muscle. These muscle lesions and ectopic stem cells are also associated with ectopic gut branches, which protrude from the normal gut towards the dorsal side of the animal. Interestingly, both megf6 and hemicentin knockdown worms are capable of regenerating tissue free of both muscle lesions and ectopic cells, indicating that these genes are dispensable for regeneration. These results provide insight into the role of planarian ECM in restricting the stem cell compartment, and suggest that signals within the compartment may act to suppress stem cell hyperproliferation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.