ArticlePloS one2020
Repeated sleep disruption in mice leads to persistent shifts in the fecal microbiome and metabolome.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
61 citing papers in PubMed, 96 citations in OpenAlex.
- Short-term sleep restriction in humans alters diurnal circulating metabolite profiles, including those of microbial origin.The Journal of clinical investigation · 2026Trial
- Insufficient sleep and weekend recovery sleep: classification by a metabolomics-based machine learning ensemble.Scientific reports · 2023Trial
- Sex and stress paradigm influence behavioural, neuroimmune, and microbiome responses in pubertal CD-1 mice.Brain, behavior, & immunity - health · 2026Article
- Voluntary wheel running behavior is reduced by SARS-CoV-2 S1 administration and mitigates hypothalamic neuroinflammatory processes.Brain, behavior, & immunity - health · 2026Article
- Stool Dynamics and the Developing Gut Microbiome During Infancy.Journal of biological rhythms · 2026Article
- From alpha diversity to zeitgebers: Functional implications and potential mechanisms linking the gut microbiome to sleep and circadian disruption (Part II).Neuroscience and biobehavioral reviews · 2026Review
- From alpha diversity to zeitgebers: Bacterial gut microbiome associations with sleep and circadian disruption (Part I).Neuroscience and biobehavioral reviews · 2026Review
- The Gut Microbiome in Sleep Disorders: A Review of Recent Evidence.Actas espanolas de psiquiatria · 2026Review
- From Gut to Brain: Probiotics as an Emerging Adjunctive Strategy for Insomnia and Sleep Disturbances.Nature and science of sleep · 2026Review
- Sleep fragmentation frequency is associated with distinct immune, microbial, and barrier-related profiles in DSS-induced colitis in mice.Frontiers in immunology · 2026Article
- Sishen wan modulates gut microbial and short-chain fatty-acid imbalances and ameliorates behavioral and inflammatory abnormalities in mice with chronic sleep deprivation.Frontiers in microbiology · 2026Article
- Linking gut microbiota rhythmicity to circadian maturation in infants.Scientific reports · 2025Article
- Microbiota metabolites affect sleep as drivers of brain‑gut communication (Review).International journal of molecular medicine · 2025Review
- Article
- The potential therapeutic approaches targeting gut health in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS): a narrative review.Journal of translational medicine · 2025Review
- Development of a preclinical model of ICU-associated sleep fragmentation and effects on pneumonia recovery in mice.American journal of physiology. Lung cellular and molecular physiology · 2025Article
- Importance of Gut Microbiota Dysbiosis and Circadian Disruption-Associated Biomarkers in Emergence of Alzheimer's Disease.Molecular neurobiology · 2025Review
- I "Gut" Rhythm: the microbiota as a modulator of the stress response and circadian rhythms.The FEBS journal · 2025Review
- Gut microbiota composition correlates with insomnia severity: insights from high-throughput sequencing analysis.Frontiers in microbiology · 2025Article
- Gut microbiota and insomnia: Mendelian randomization and network pharmacology to predict potential intervention with traditional Chinese medicine.Archives of medical science : AMS · 2025Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
It has been established in recent years that the gut microbiome plays a role in health and disease, potentially via alterations in metabolites that influence host physiology. Although sleep disruption and gut dysbiosis have been associated with many of the same diseases, studies investigating the gut microbiome in the context of sleep disruption have yielded inconsistent results, and have not assessed the fecal metabolome. We exposed mice to five days of sleep disruption followed by four days of ad libitum recovery sleep, and assessed the fecal microbiome and fecal metabolome at multiple timepoints using 16S rRNA gene amplicons and untargeted LC-MS/MS mass spectrometry. We found global shifts in both the microbiome and metabolome in the sleep-disrupted group on the second day of recovery sleep, when most sleep parameters had recovered to baseline levels. We observed an increase in the Firmicutes:Bacteroidetes ratio, along with decreases in the genus Lactobacillus, phylum Actinobacteria, and genus Bifidobacterium in sleep-disrupted mice compared to control mice. The latter two taxa remained low at the fourth day post-sleep disruption. We also identified multiple classes of fecal metabolites that were differentially abundant in sleep-disrupted mice, some of which are physiologically relevant and commonly influenced by the microbiome. This included bile acids, and inference of microbial functional gene content suggested reduced levels of the microbial bile salt hydrolase gene in sleep-disrupted mice. Overall, this study adds to the evidence base linking disrupted sleep to the gut microbiome and expands it to the fecal metabolome, identifying sleep disruption-sensitive bacterial taxa and classes of metabolites that may serve as therapeutic targets to improve health after poor sleep.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.