ArticleMolecular biology and evolution2020
Doublesex Mediates the Development of Sex-Specific Pheromone Organs in Bicyclus Butterflies via Multiple Mechanisms.
Article in Molecular biology and evolution, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 36 citations in OpenAlex.
- Butterfly brains change in morphology and in gene splicing patterns after brief pheromone exposure.BMC biology · 2026Article
- Single-nucleus transcriptomics of wing sexual dimorphism and scale cell specialization in sulphur butterflies.PLoS biology · 2025Article
- Transcriptomic Evidence for Cell-Autonomous Sex Differentiation of the Gynandromorphic Fat Body in the Silkworm,Journal of developmental biology · 2024Article
- Novel Sex-Specific Genes and Diverse Interspecific Expression in the Antennal Transcriptomes of Ithomiine Butterflies.Genome biology and evolution · 2024Article
- CRISPR/Cas9-Based Genome Editing of Fall Armyworm (Biomolecules · 2024Review
- Zygosity-based sex determination in a butterfly drives hypervariability ofScience advances · 2024Article
- CRISPR/Cas9-Mediated Mutagenesis of Sex-SpecificCells · 2022Article
- Secondary reversion to sexual monomorphism associated with tissue-specific loss of doublesex expression.Evolution; international journal of organic evolution · 2022Article
- A genetic switch for male UV iridescence in an incipient species pair of sulphur butterflies.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Novel Doublesex Duplication Associated with Sexually Dimorphic Development of Dogface Butterfly Wings.Molecular biology and evolution · 2021Article
- Stage- and sex-specific transcriptome analyses reveal distinctive sensory gene expression patterns in a butterfly.BMC genomics · 2021Article
- Evolution of sexual development and sexual dimorphism in insects.Current opinion in genetics & development · 2021Review
- Article
- Evolutionary trade-offs between male secondary sexual traits revealed by a phylogeny of the hyperdiverse tribe Eumaeini (Lepidoptera: Lycaenidae).Proceedings. Biological sciences · 2021Article
- Hox genes are essential for the development of eyespots in Bicyclus anynana butterflies.Genetics · 2021Article
- Rapid parallel adaptation despite gene flow in silent crickets.Nature communications · 2021Article
- Transgenic and knockout analyses of Masculinizer and doublesex illuminated the unique functions of doublesex in germ cell sexual development of the silkworm, Bombyx mori.BMC developmental biology · 2020Article
- Dissection of Larval and Pupal Wings ofMethods and protocols · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Bicyclus lineage of satyrid butterflies exhibits male-specific traits, the scent organs, used for chemical communication during courtship. These organs consist of tightly packed brush-like scales (hair-pencils) that rub against scent patches to disperse pheromones, but the evolution and molecular basis of these organ's male-limited development remains unknown. Here, we examine the evolution of the number and location of the scent patches and hair-pencils within 53 species of Bicyclus butterflies, and the involvement of the sex determinant gene doublesex (dsx) in scent organ development in Bicyclus anynana using CRISPR/Cas9. We show that scent patches and hair-pencils arose via multiple, independent gains, in a correlated manner. Further, an initially nonsex-specific Dsx protein expression pattern in developing wing discs becomes male-specific and spatially refined to areas that develop the scent patches. Functional perturbations of dsx show that this gene activates patch development in males whereas hair-pencils develop in both sexes without Dsx input. Dsx in females is, instead, required to repress hair-pencils whereas Dsx in males regulates minor aspects of its development. These findings suggest that the patches and hair-pencils evolve as correlated composite organs presumably due to their functional integration. Divergence in the function of dsx isoforms occurred across the sexes, where the male isoform promotes patch development in males and the female isoform represses hair-pencil development in females, both leading to the development of male-limited traits. Furthermore, evolution in number of patches in males is due to the evolution of spatial regulation of dsx.
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