ArticlePest management science2020
A standardised bioassay method using a bench-top spray tower to evaluate entomopathogenic fungi for control of the greenhouse whitefly, Trialeurodes vaporariorum.
Article in Pest management science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Sustainable management of whitefly (Bemisia tabaci) in vegetables using entomopathogenic fungi, Isaria farinosa: Characterization, efficacy, insecticide compatibility with molecular docking insights.World journal of microbiology & biotechnology · 2026Article
- Monoterpene-Rich Nanoemulsion fromMolecules (Basel, Switzerland) · 2026Article
- Improved control of Trialeurodes vaporariorum using mixture combinations of entomopathogenic fungi and the chemical insecticide spiromesifen.Scientific reports · 2024Article
- Article
- Entomopathogenic Fungi as a Potential Management Tool for the Control of Urban Malaria Vector,Journal of fungi (Basel, Switzerland) · 2023Article
- Efficacy ofFrontiers in insect science · 2022Article
- Effectiveness of Entomopathogenic Fungi on Immature Stages and Feeding Performance of Fall Armyworm,Insects · 2021Article
- Endophytic Colonisation ofFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
Abstract
backgroundBioassays evaluating entomopathogenic fungi (EPF) isolates for effective microbial control of whitefly are a fundamental part of the screening process for bioprotectants, but development of repeatable, robust bioassays is not straightforward. Currently, there is no readily available standardised method to test the efficacy of EPF on whitefly. Here, we describe the calibration and use of a spray tower to deliver a standardised protocol to assess EPF activity; the method was validated using 18 EPF from four genera in tests against greenhouse whitefly, Trialeurodes vaporariorum (Westwood).
resultsAt 138 kPa, the sprayer delivered 0.062 mL mm
conclusionCombining the calibrated sprayer and bioassay method provides a reliable, standardised approach to test the virulence of EPF against whitefly nymphs. This laboratory-based assay is affordable, replicable and allows the user to alter the dose of conidia applied to the target.
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