ArticleMikrochimica acta2020
Simultaneously responsive microfluidic chip aptasensor for determination of kanamycin, aflatoxin M1, and 17β-estradiol based on magnetic tripartite DNA assembly nanostructure probes.
Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Perspectives in Aptasensor-Based Portable Detection for Biotoxins.Molecules (Basel, Switzerland) · 2024Review
- Revealing an initiation inhibition of RCA and its application in nucleic acid detection.Acta biochimica et biophysica Sinica · 2023Article
- Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review.Toxins · 2022Review
- Aptamer-based assays: strategies in the use of aptamers conjugated to magnetic micro- and nanobeads as recognition elements in food control.Analytical and bioanalytical chemistry · 2022Article
- Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk.Micromachines · 2021Review
- Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs.Molecules (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The authors describe a microfluidic chip-based aptasensor platform combined with magnetic tripartite DNA structure-functionalized nanocomposites to achieve simultaneous determination of kanamycin (KANA), aflatoxin M1 (AFM1), and 17β-estradiol (E2) in milk. The two-duplex tripartite DNA nanostructure was first assembled on the surface of magnetic beads. When the aptamer on the probes recognized the specific target, the aptamer-target would be released into the supernatant. The pre-primer@circular DNA template structure initiates rolling circle amplification (RCA) by phi29 polymerase. After magnetic separation, the magnetic nanocomposites were added into a solution containing three different lengths of complementary strands to the RCA products. The number of complementary strands significantly decrease, and this can be quantitated by the microfluidic chip. Further, the employment of magnetic nanocomposites and microfluidic chip not only resolve the complex matrix interference, but also dramatically enhances the determination selectivity and sensitivity. This aptasensor allows for determination of KANA, AFM1, and E2 with limits of detection as low as 0.32 pg mL
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Registered trials
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