ArticlePloS one2020
Callus growth kinetics and accumulation of secondary metabolites of Bletilla striata Rchb.f. using a callus suspension culture.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 58 citations in OpenAlex.
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- Cytochemical localization and synthesis mechanism of the glucomannan in pseudobulbs ofHorticulture research · 2024Article
- The appearance, active components, antioxidant activities, and their markers in fibrous roots of Bletilla striata.PloS one · 2024Article
- Age-induced Changes in Ginsenoside Accumulation and Primary Metabolic Characteristics of Panax Ginseng in Transplantation Mode.Journal of ginseng research · 2024Article
- Insights into Metabolic Engineering of Bioactive Molecules inCurrent genomics · 2023Review
- Identification, Biological Function Profiling and Biosynthesis of Secondary Metabolites in Medicinal Orchids.Metabolites · 2023Review
- Extract of Bletilla formosana callus elevates cellular antioxidative activity via Nrf2/HO-1 signaling pathway and inhibits melanogenesis in zebrafish.Journal, genetic engineering & biotechnology · 2023Article
- In vitro strategies for the enhancement of secondary metabolite production in plants: a review.Bulletin of the National Research Centre · 2022Review
- Logistic modeling to predict the minimum inhibitory concentration (MIC) of olive leaf extract (OLE) against Listeria monocytogenes.PloS one · 2022Article
- Nanoparticles induce genetic, biochemical, and ultrastructure variations in Salvadora persica callus.Journal, genetic engineering & biotechnology · 2021Article
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- Metabolic Profiling of Primary Metabolites and Galantamine Biosynthesis in WoundedPlants (Basel, Switzerland) · 2020Article
- Full Transcriptome Analysis of Callus Suspension Culture System ofFrontiers in genetics · 2020Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bletilla striata is an endangered traditional Chinese medicinal plant with multiple uses and a slow regeneration rate of its germplasm resources. To evaluate the callus growth kinetics and accumulation of secondary metabolites (SMs), a callus suspension culture was proven to be a valuable approach for acquiring high yields of medicinal compounds. An effective callus suspension culture for obtaining B. striata callus growth and its SMs was achieved with the in vitro induction of calluses from B. striata seeds. The callus growth kinetics and accumulation of SMs were analyzed using a mathematical model. The resulting callus growth kinetic model revealed that the growth curves of B. striata suspension-cultured calluses were sigmoidal, indicating changes in the growth of the suspension-cultured calluses. Improved Murashige and Skoog callus growth medium was the most favorable medium for B. striata callus formation, with the highest callus growth occurring during the stationary phase of the cultivation period. Callus growth acceleration started after 7 days and thereafter gradually decreased until day 24 of the cultivation period and reached its highest at day 36 period in both the dry weight and fresh weight analyses. The coelonin concentration peaked during the exponential growth stage and decreased afterward during the stationary stage of the callus suspension culture. The maximum content of coelonin (approximately 0.3323 mg/g callus dry weight) was observed on the 18th day of the cultivation cycle, while dactylorhin A and militarine reached the highest concentrations at day 24, and p-hydroxybenzyl alcohol at day 39. This investigation also laid a foundation for a multimathematical model to better describe the accumulation variation of SMs. The production of SMs showed great specificity during callus growth and development. This research provided a well-organized way to increase the accumulation and production of SMs during the scaled-up biosynthesis of calluses in B. striata callus suspension cultures.
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