ArticleeLife2020
Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 43 citations in OpenAlex.
- Antisense oligonucleotide-mediated disruption of mRNA localisation inhibits angiogenesis.Angiogenesis · 2026Article
- Advances in Optical Tissue Clearing for Three-Dimensional Ocular Imaging and Analysis.Journal of ophthalmology · 2026Review
- Rapid 3D Immunolabeling and Light Sheet Microscopy for Quantitative Analysis of Intact Tissues.Computational and structural biotechnology journal · 2026Article
- Optimizing tissue clearing methods for improved imaging of whole-mount retinas and optic nerves.Journal of neuroscience methods · 2025Article
- Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Review
- Association of triglyceride glucose index with diabetic retinopathy in middle-aged and elderly ambulatory type 2 diabetic patients.Frontiers in endocrinology · 2025Article
- Collagen crosslinking-induced corneal morphological changes: a three-dimensional light sheet Microscopy-based evaluation.Scientific reports · 2024Article
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- 3DVascNet: An Automated Software for Segmentation and Quantification of Mouse Vascular Networks in 3D.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- Expanding the Horizons of Pre-Transplant Renal Vascular Assessment Using Ex Vivo Perfusion.Current issues in molecular biology · 2023Review
- Detection of Choroidal Neovascularization Using Optical Tissue Transparency.Translational vision science & technology · 2023Article
- Article
- A workflow to visualize vertebrate eyes in 3D.PloS one · 2023Article
- Article
- Combining multiple fluorescence imaging techniques in biology: when one microscope is not enough.Molecular biology of the cell · 2022Article
- Longitudinal dynamics of microvascular recovery after acquired cortical injury.Acta neuropathologica communications · 2022Article
- Microscopic Imaging Methods for Organ-on-a-Chip Platforms.Micromachines · 2022Review
- Endothelial cells during craniofacial development: Populating and patterning the head.Frontiers in bioengineering and biotechnology · 2022Review
- Light-Sheet Microscopy of the Optic Nerve Reveals Axonal Degeneration and Microglial Activation in NMDA-Induced Retinal Injury.EC ophthalmology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 7 institutions in 4 countries.
Funding
Abstract
As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models. Here, we demonstrate that 1) rapid, quantitative 3D and 4D (time lapse) imaging of cellular and subcellular processes in the mouse eye is feasible, with and without tissue clearing, using light-sheet fluorescent microscopy (LSFM); 2) flat-mounting retinas for confocal microscopy significantly distorts tissue morphology, confirmed by quantitative correlative LSFM-Confocal imaging of vessels; 3) LSFM readily reveals new features of even well-studied eye disease mouse models, such as the oxygen-induced retinopathy (OIR) model, including a previously unappreciated 'knotted' morphology to pathological vascular tufts, abnormal cell motility and altered filopodia dynamics when live-imaged. We conclude that quantitative 3D/4D LSFM imaging and analysis has the potential to advance our understanding of the eye, in particular pathological, neurovascular, degenerative processes.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.