ReviewJournal of microscopy2020
Multiphoton intravital microscopy in small animals: motion artefact challenges and technical solutions.
Review in Journal of microscopy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Bibliometric analysis of research hotspots and trends in fascia from 2013 to 2025.Surgical and radiologic anatomy : SRA · 2026Review
- Multimodal optical imaging strategy to evaluate the microscopic biodistribution of SERS gold nanoparticles across histology sections.Biophotonics discovery · 2026Article
- Quantification of Lacteal Contractility by Live Intravital Imaging.Microcirculation (New York, N.Y. : 1994) · 2026Article
- Serial intravital microscopy reveals temporal dynamics of autoreactive germinal centers in the spleen.iScience · 2026Article
- Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging.Pathology international · 2025Review
- Understanding the nervous system: lessons from Frontiers in Neurophotonics.Neurophotonics · 2024Review
- Subcellular analysis of blood-brain barrier function by micro-impalement of vessels in acute brain slices.Nature communications · 2023Article
- Serial intravital 2-photon microscopy and analysis of the kidney using upright microscopes.Frontiers in physiology · 2023Article
- Spectral Multiplexing of Fluorescent Endoscopy for Simultaneous Imaging with Multiple Fluorophores and Multiple Fields of View.Biosensors · 2022Article
- Live imaging of platelets and neutrophils during antibody-mediated neurovascular thrombosis.Blood advances · 2022Article
- Monitoring Immune Cell Function Through Optical Imaging: a Review Highlighting Transgenic Mouse Models.Molecular imaging and biology · 2022Review
- Advanced Tumor Imaging Approaches in Human Tumors.Cancers · 2022Review
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Fluorescence Microscopy-An Outline of Hardware, Biological Handling, and Fluorophore Considerations.Cells · 2021Review
- 3D Whole-Brain Imaging Approaches to Study Brain Tumors.Cancers · 2021Review
- Digital Image Analysis Tools Developed by the Indiana O'Brien Center.Frontiers in physiology · 2021Review
- Review
- Label-Free Multiphoton Microscopy for the Detection and Monitoring of Calcific Aortic Valve Disease.Frontiers in cardiovascular medicine · 2021Review
- Machine Learning Aided Photonic Diagnostic System for Minimally Invasive Optically Guided Surgery in the Hepatoduodenal Area.Diagnostics (Basel, Switzerland) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Since its invention 29 years ago, two-photon laser-scanning microscopy has evolved from a promising imaging technique, to an established widely available imaging modality used throughout the biomedical research community. The establishment of two-photon microscopy as the preferred method for imaging fluorescently labelled cells and structures in living animals can be attributed to the biophysical mechanism by which the generation of fluorescence is accomplished. The use of powerful lasers capable of delivering infrared light pulses within femtosecond intervals, facilitates the nonlinear excitation of fluorescent molecules only at the focal plane and determines by objective lens position. This offers numerous benefits for studies of biological samples at high spatial and temporal resolutions with limited photo-damage and superior tissue penetration. Indeed, these attributes have established two-photon microscopy as the ideal method for live-animal imaging in several areas of biology and have led to a whole new field of study dedicated to imaging biological phenomena in intact tissues and living organisms. However, despite its appealing features, two-photon intravital microscopy is inherently limited by tissue motion from heartbeat, respiratory cycles, peristalsis, muscle/vascular tone and physiological functions that change tissue geometry. Because these movements impede temporal and spatial resolution, they must be properly addressed to harness the full potential of two-photon intravital microscopy and enable accurate data analysis and interpretation. In addition, the sources and features of these motion artefacts are varied, sometimes unpredictable and unique to specific organs and multiple complex strategies have previously been devised to address them. This review will discuss these motion artefacts requirement and technical solutions for their correction and after intravital two-photon microscopy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.