ArticleNature communications2020
miRNA-1-3p is an early embryonic male sex-determining factor in the Oriental fruit fly Bactrocera dorsalis.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- A Comparative Transcriptomic Analysis of X-Ray Irradiation Responses Identifies Candidate Biomarker Genes inInsects · 2026Article
- Environmental sex determination in the cyst nematode Globodera pallida defaults to male development.BMC biology · 2026Article
- Review
- Thermal Plasticity is Regulated by a Key MicroRNA During Range Expansion of an Invasive Fruit Fly.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The Potential Risk ofInsects · 2025Review
- Integrated Analysis of Differential Expression Profiles of miRNA and mRNA in Gonads ofAnimals : an open access journal from MDPI · 2025Article
- miR-NPs-RVG promote spinal cord injury repair: implications from spinal cord-derived microvascular endothelial cells.Journal of nanobiotechnology · 2024Article
- microRNA-1 regulates sea urchin skeletogenesis by directly targeting skeletogenic genes and modulating components of signaling pathways.Developmental biology · 2024Article
- Identification of testis development-related genes by combining Iso-Seq and RNA-Seq inFrontiers in cell and developmental biology · 2024Article
- The genome and sex-dependent responses to temperature in the common yellow butterfly, Eurema hecabe.BMC biology · 2023Article
- Identification and Characterization of Development-Related microRNAs in the Red Flour Beetle,International journal of molecular sciences · 2023Article
- Systematic Characterization and Regulatory Role of lncRNAs in Asian Honey Bees Responding to Microsporidian Infestation.International journal of molecular sciences · 2023Article
- MiRNA Profiling and Its Potential Roles in Rapid Growth of Velvet Antler in Gansu Red Deer (Genes · 2023Article
- The identification and expression pattern of the sex determination genes and their sex-specific variants in the egg parasitoidFrontiers in physiology · 2023Article
- miR-275/305 cluster is essential for maintaining energy metabolic homeostasis by the insulin signaling pathway in Bactrocera dorsalis.PLoS genetics · 2022Article
- Two Short Repeats in the 5' Untranslated Region of Insulin-like Androgenic Gland Factor inInternational journal of molecular sciences · 2022Article
- Identification and Characterization of MicroRNAs Involving in Initial Sex Differentiation ofBiology · 2022Article
- Evolution of sexual development and sexual dimorphism in insects.Current opinion in genetics & development · 2021Review
- Downregulation of miR-3934 in Peripheral Blood Mononuclear Cells of Asthmatic Patients and Its Potential Diagnostic Value.BioMed research international · 2021Article
- The transformer-2 and fruitless characterisation with developmental expression profiles of sex-determining genes in Bactrocera dorsalis and B. correcta.Scientific reports · 2020Article
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
Regulation of male sexual differentiation by a Y chromosome-linked male determining factor (M-factor) is one of a diverse array of sex determination mechanisms found in insects. By deep sequencing of small RNAs from Bactrocera dorsalis early embryos, we identified an autosomal-derived microRNA, miR-1-3p, that has predicted target sites in the transformer gene (Bdtra) required for female sex determination. We further demonstrate by both in vitro and in vivo tests that miR-1-3p suppresses Bdtra expression. Injection of a miR-1-3p mimic in early embryos results in 87-92% phenotypic males, whereas knockdown of miR-1-3p by an inhibitor results in 67-77% phenotypic females. Finally, CRISPR/Cas9-mediated knockout of miR-1-3p results in the expression of female-specific splice variants of Bdtra and doublesex (Bddsx), and induced sex reversal of XY individuals into phenotypic females. These results indicate that miR-1-3p is required for male sex determination in early embryogenesis in B. dorsalis as an intermediate male determiner.
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