ArticleProceedings of the National Academy of Sciences of the United States of America2020
Spatial mapping of tissue properties in vivo reveals a 3D stiffness gradient in the mouse limb bud.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 85 citations in OpenAlex.
- Organoid Intelligent Morphomics: Decoding the organoid morphome through artificial intelligence from phenotypic quantification to mechanistic insight.Bioactive materials · 2027Review
- Prepatterned Tissue Stiffness Gradient Controls Organ shape and size.bioRxiv : the preprint server for biology · 2026Article
- Failure of morphogenesis in chronic inflammation-associated early tumourigenesis: Extracellular matrix biomechanics as an integrative layer.Biochemistry and biophysics reports · 2026Review
- Drop-Shaped Optical Microfiber Enabled Biomechanical Sensor.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Tumor Development in Ulcerative Colitis: Perspectives From Biomechanical Characteristics.Development, growth & differentiation · 2025Review
- Dynamic rigidity changes enable rapid cell migration on soft substrates.Nature communications · 2025Article
- A method for analysing tissue motion and deformation during mammalian organogenesis.PLoS computational biology · 2025Article
- A thermodynamic perspective on mammalian neural crest ingression.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Durotaxis is a driver and potential therapeutic target in lung fibrosis and metastatic pancreatic cancer.Nature cell biology · 2025Article
- Tissue stiffness mapping by light sheet elastography.Science advances · 2025Article
- The first embryo, the origin of cancer and animal phylogeny. V. Cancer stem cells as the unifying biomechanical principle between embryology and oncology.Mechanobiology in medicine · 2025Review
- Probabilistic analysis of spatial viscoelastic cues in 3D cell culture using magnetic microrheometry.Biophysical journal · 2025Article
- siRNA Treatment Enhances Collagen Fiber Formation in Tissue-Engineered Meniscus via Transient Inhibition of Aggrecan Production.Bioengineering (Basel, Switzerland) · 2024Article
- Lamellipodia-Mediated Osteoblast Haptotaxis Guided by Fibronectin Ligand Concentrations on a Multiplex Chip.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Spatial regulation of hydrogel polymerization reaction using ultrasound-driven streaming vortex.Ultrasonics sonochemistry · 2024Article
- A fibronectin gradient remodels mixed-phase mesoderm.Science advances · 2024Article
- Mesenchymal Wnts are required for morphogenetic movements of calvarial osteoblasts during apical expansion.Development (Cambridge, England) · 2024Article
- The first embryo, the origin of cancer and animal phylogeny. III. The totipotency as revealed by morphogenesis and the neoplasia controlled by cellular differentiation.Journal of biosciences · 2024Review
- Biophysical control of plasticity and patterning in regeneration and cancer.Cellular and molecular life sciences : CMLS · 2023Review
- Differential cell motion: A mathematical model of anterior posterior sorting.Biophysical journal · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Numerous hypotheses invoke tissue stiffness as a key parameter that regulates morphogenesis and disease progression. However, current methods are insufficient to test hypotheses that concern physical properties deep in living tissues. Here we introduce, validate, and apply a magnetic device that generates a uniform magnetic field gradient within a space that is sufficient to accommodate an organ-stage mouse embryo under live conditions. The method allows rapid, nontoxic measurement of the three-dimensional (3D) spatial distribution of viscoelastic properties within mesenchyme and epithelia. Using the device, we identify an anteriorly biased mesodermal stiffness gradient along which cells move to shape the early limb bud. The stiffness gradient corresponds to a
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.