Evidence map›Paper›PMID 32071071›Full record

ArticleG3 (Bethesda, Md.)2020

BlobToolKit - Interactive Quality Assessment of Genome Assemblies.

Richard Challis, Edward Richards, Jeena Rajan, Guy Cochrane, Mark Blaxter

Abstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1,633 papers.

0numbers the graph read from it
0cells of the map it votes in
1,633citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1,633 citing papers in PubMed.

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  20. Programmed DNA elimination was present in the last common ancestor ofbioRxiv : the preprint server for biology · 2026
    Article

1,573 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Richard ChallisInstitute of Evolutionary Biology, University of Edinburgh, Edinburgh EH9 3JT, UK rc28@sanger.ac.uk.ORCID 0000-0002-3502-1122
Edward RichardsEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Cambridge CB10 1SD, UK.ORCID 0000-0001-5975-6003
Jeena RajanEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Cambridge CB10 1SD, UK.ORCID 0000-0002-3613-0013
Guy CochraneEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Cambridge CB10 1SD, UK.ORCID 0000-0001-7954-7057
Mark BlaxterInstitute of Evolutionary Biology, University of Edinburgh, Edinburgh EH9 3JT, UK.ORCID 0000-0003-2861-949X

Funding

Biotechnology and Biological Sciences Research Council BB/P024238/1
6 · The paper itself

Abstract

Reconstruction of target genomes from sequence data produced by instruments that are agnostic as to the species-of-origin may be confounded by contaminant DNA. Whether introduced during sample processing or through co-extraction alongside the target DNA, if insufficient care is taken during the assembly process, the final assembled genome may be a mixture of data from several species. Such assemblies can confound sequence-based biological inference and, when deposited in public databases, may be included in downstream analyses by users unaware of underlying problems. We present BlobToolKit, a software suite to aid researchers in identifying and isolating non-target data in draft and publicly available genome assemblies. BlobToolKit can be used to process assembly, read and analysis files for fully reproducible interactive exploration in the browser-based Viewer. BlobToolKit can be used during assembly to filter non-target DNA, helping researchers produce assemblies with high biological credibility. We have been running an automated BlobToolKit pipeline on eukaryotic assemblies publicly available in the International Nucleotide Sequence Data Collaboration and are making the results available through a public instance of the Viewer at https://blobtoolkit.genomehubs.org/view We aim to complete analysis of all publicly available genomes and then maintain currency with the flow of new genomes. We have worked to embed these views into the presentation of genome assemblies at the European Nucleotide Archive, providing an indication of assembly quality alongside the public record with links out to allow full exploration in the Viewer.

Indexed as

GenomeSoftwareSequence Analysis, DNABioinformaticsgenome assemblyquality controlvisualisation web-tool

Identifiers

PMID32071071
PMCPMC7144090

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.