ArticleBMC genomics2020
A multiplex PCR assay for rapid identification of major tospovirus vectors reported in India.
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Multiplex PCR for the differentiation ofCurrent research in parasitology & vector-borne diseases · 2026Article
- Multiplex PCR-based identification of two sympatric stem borer species, Sesamia cretica and Sesamia nonagrioides (Lepidoptera: Noctuidae).Journal of insect science (Online) · 2025Article
- Development of a Polymerase Spiral Reaction-Based Isothermal Assay for Rapid Identification ofFrontiers in molecular biosciences · 2022Article
- Groundnut Bud Necrosis Virus Modulates the Expression of Innate Immune, Endocytosis, and Cuticle Development-Associated Genes to Circulate and Propagate in Its Vector,Frontiers in microbiology · 2022Article
- Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.PloS one · 2022Article
- Review
- Article
- A rapid field-based assay using recombinase polymerase amplification for identification ofJournal of pest science · 2021Article
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Authors and funding
5 authors.
Funding
Abstract
backgroundTo date, four thrips vectors have been reported to transmit five different tospoviruses in India. Their identification at an early stage is crucial in formulating appropriate pest management strategies. Since morphometric key-based thrips identification based on the adult stage is time-consuming, there is a need to develop diagnostic tools which are rapid, accurate, and independent of developmental stages. Here, we report a multiplex PCR assay to identify four major thrips vectors viz. Thrips palmi, T. tabaci, Scirtothrips dorsalis, and Frankliniella schultzei present in India.
resultsCytochrome oxidase subunit III and internal transcribed spacer region 2 were utilized to design species-specific primers. Of 38 pairs of primers tested, primer pairs AG35F-AG36R, AG47F-AG48R, AG87F-AG88R, and AG79F-AG80R amplified 568 bp, 713 bp, 388 bp, and 200 bp products from the DNA templates of T. palmi, S. dorsalis, T. tabaci, and F. schultzei, respectively at same PCR conditions. The specificity of the primer pairs was validated with a large number of known specimens and no cross-reactivity was observed with other thrips species. The multiplex PCR assay with a cocktail of all the four primer pairs detected four thrips vectors efficiently and could discriminate all of them concurrently in a single reaction.
conclusionThe multiplex PCR reported in this study could identify the major thrips vectors reported in India. The assay will be useful in ascertaining distribution profile of major thrips vectors, disease epidemiology, screening large samples, and quarantine.
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