Evidence map›Paper›PMID 32070230›Full record

ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2020

Guidelines for Sanger sequencing and molecular assay monitoring.

Beate M Crossley, Jianfa Bai, Amy Glaser, Roger Maes, Elizabeth Porter, Mary Lea Killian, Travis Clement, Kathy Toohey-Kurth

Open access · bronzeAbstract read
In one paragraph

Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
112citing papers in PubMed, 1 pooled it
9.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

112 citing papers in PubMed, 1 synthesis or guideline pooled it, 247 citations in OpenAlex.

  1. No association ofFrontiers in neurology · 2022
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  4. Short-Read NGS of a Long-RangeInternational journal of molecular sciences · 2026
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  10. Novel Prion Protein Gene (bioRxiv : the preprint server for biology · 2026
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  17. PhytoKeys · 2026
    Article
  18. Macrolide resistance trajectories across threeFrontiers in microbiology · 2026
    Article
  19. Review
  20. Article

52 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Beate M CrossleyCalifornia Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Crossley, Toohey-Kurth).ORCID https://orcid.org/0000-0003-2932-7229
Jianfa BaiCalifornia Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Crossley, Toohey-Kurth).
Amy GlaserCalifornia Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Crossley, Toohey-Kurth).
Roger MaesCalifornia Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Crossley, Toohey-Kurth).
Elizabeth PorterCalifornia Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Crossley, Toohey-Kurth).
Mary Lea KillianCalifornia Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Crossley, Toohey-Kurth).
Travis ClementCalifornia Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Crossley, Toohey-Kurth).
Kathy Toohey-KurthCalifornia Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Crossley, Toohey-Kurth).
South Dakota State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetic sequencing, or DNA sequencing, using the Sanger technique has become widely used in the veterinary diagnostic community. This technology plays a role in verification of PCR results and is used to provide the genetic sequence data needed for phylogenetic analysis, epidemiologic studies, and forensic investigations. The Laboratory Technology Committee of the American Association of Veterinary Laboratory Diagnosticians has prepared guidelines for sample preparation, submission to sequencing facilities or instrumentation, quality assessment of nucleic acid sequence data performed, and for generating basic sequencing data and phylogenetic analysis for diagnostic applications. This guidance is aimed at assisting laboratories in providing consistent, high-quality, and reliable sequence data when using Sanger-based genetic sequencing as a component of their laboratory services.

Indexed as

Animal DiseasesAnimalsBase SequenceHigh-Throughput Nucleotide SequencingHumansLaboratoriesPhylogenyPolymerase Chain ReactionSequence Analysis, DNAquality scoreSanger sequencingvalidation

Identifiers

PMID32070230
PMCPMC7649556
OpenAlexW3006789725

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.