Evidence map›Paper›PMID 32069774›Full record

ArticleMolecular therapy. Nucleic acids2020

MSC-Secreted Exosomal H19 Promotes Trophoblast Cell Invasion and Migration by Downregulating let-7b and Upregulating FOXO1.

Yang Chen, Haiyan Ding, Min Wei, Wenhui Zha, Shuang Guan, Ning Liu, Yang Li, Yuan Tan, Yan Wang, Fuju Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy. Nucleic acids, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 72 citations in OpenAlex.

  1. Article
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  8. Frontiers in cellular and infection microbiology · 2025
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  9. Review
  10. Article
  11. Extracellular Vesicles: the Next Frontier in Pregnancy Research.Reproductive sciences (Thousand Oaks, Calif.) · 2024
    Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Review
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Yang ChenDepartment of Gynecology and Obstetrics, The Second Hospital of Jilin University, Changchun 130041, P.R. China.
Haiyan DingDepartment of Gynecology and Obstetrics, The Second Hospital of Jilin University, Changchun 130041, P.R. China.
Min WeiDepartment of Gynecology and Obstetrics, The Second Hospital of Jilin University, Changchun 130041, P.R. China.
Wenhui ZhaDepartment of Gynecology and Obstetrics, The Second Hospital of Jilin University, Changchun 130041, P.R. China.
Shuang GuanDepartment of Rehabilitation, The Second Hospital of Jilin University, Changchun 130041, P.R. China.
Ning LiuDepartment of Gynecology and Obstetrics, The Second Hospital of Jilin University, Changchun 130041, P.R. China.
Yang LiCenter of Reproductive Medicine, Center of Prenatal Diagnosis, The First Hospital of JiLin University, Changchun 130041, P.R. China.
Yuan TanDepartment of Gynecology and Obstetrics, The Second Hospital of Jilin University, Changchun 130041, P.R. China.
Yan WangDepartment of Gynecology and Obstetrics, The Second Hospital of Jilin University, Changchun 130041, P.R. China.
Fuju WuDepartment of Gynecology and Obstetrics, The Second Hospital of Jilin University, Changchun 130041, P.R. China. Electronic address: fujuwu0052@sina.com.
Second Affiliated Hospital of Jilin University · CNFirst Hospital of Jilin University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosomes perform important functions for intercellular communication through extracellular signaling pathways, leading to the regulation of important biological processes, including cell proliferation, but also systemic dysfunctions such as preeclampsia (PE). However, the inhibitory effects of mesenchymal stem cell (MSCs)-derived exosomes in PE remain largely unknown. Thus, we assessed the possibility that exosomes could transport long non-coding RNA H19 and the correlation between H19 and the apoptosis of trophoblast cells. The expression of microRNA let-7b and forkhead box protein O1 (FOXO1) was characterized in placental tissues of PE patients. Gain- and loss-of-function experiments were performed to examine the roles of FOXO1 and let-7b in trophoblast cells. Interactions between let-7b and H19 as well as between let-7b and FOXO1 were confirmed by a dual-luciferase reporter assay, RNA pull-down, and RNA immunoprecipitation. HTR-8/SVneo cells were co-cultured with exosomes derived from MSCs overexpressing H19, followed by invasion, migration, and apoptosis assessments of trophoblast cells. We found that let-7b was highly expressed and FOXO1 was poorly expressed in placental tissues of PE patients. Furthermore, H19 acts as a competitive endogenous RNA against let-7b, and let-7b directly targeted FOXO1. Moreover, H19 could be transferred to trophoblast cells via MSC-secreted exosomes. MSC-derived exosomes overexpressing H19 decreased let-7b, increased FOXO1, and activated the protein kinase B (AKT) signaling pathway, thus increasing invasion and migration and inhibiting apoptosis of trophoblast cells. These results suggest that MSC-derived exosomes overexpressing H19 may be a novel direction for therapeutic strategies against PE.

Indexed as

AKT signaling pathwayexosomesFOXO1let-7blong non-coding RNA H19mesenchymal stem cellspreeclampsia

Identifiers

PMID32069774
PMCPMC7026285
OpenAlexW2994540922

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.