ArticleAnnals of laboratory medicine2020
Determination of Colistin Resistance by Simple Disk Diffusion Test Using Modified Mueller-Hinton Agar.
Article in Annals of laboratory medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 26 citations in OpenAlex.
- Comprehensive genomic characterization and public health implications of multidrug-resistant Escherichia coli from poultry in Noakhali, Bangladesh.BMC microbiology · 2026Article
- Analysis of pathogen distribution and association with lipid levels in patients with diabetic foot.Frontiers in medicine · 2026Article
- Dynamics understanding of novel solvated drug molecules against emergingResults in chemistry · 2025Article
- Public health threat of antimicrobial resistance and virulence genes in Escherichia coli from human-chicken transmission in Egypt.Scientific reports · 2025Article
- Multi-drug resistant (MDR) Gram-negative pathogenic bacteria isolated from poultry in the Noakhali region of Bangladesh.PloS one · 2024Article
- Synergistic Antimicrobial Effects of Ibuprofen Combined with Standard-of-Care Antibiotics against Cystic Fibrosis Pathogens.Biomedicines · 2023Article
- Extended-spectrum β-lactamase- producing gram-negative bacterial infections in severely ill COVID-19 patients admitted in a national referral hospital, Kenya.Annals of clinical microbiology and antimicrobials · 2023Article
- Mutation of putative glycosyl transferases PslC and PslI confers susceptibility to antibiotics and leads to drastic reduction in biofilm formation inMicrobiology (Reading, England) · 2023Article
- Multidrug resistant bacterial infections in severely ill COVID-19 patients admitted in a national referral and teaching hospital, Kenya.BMC infectious diseases · 2022Article
- In Vitro Antimicrobial Activity of the Siderophore Cephalosporin Cefiderocol againstAntibiotics (Basel, Switzerland) · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundColistin has become a last-resort antibiotic for the management of multidrug-resistant gram-negative bacteria. The disk diffusion test is cheap and easy to perform but may be unreliable for colistin susceptibility testing due to poor diffusion of the large colistin molecule. An improved agar diffusion test would increase the reliability of colistin susceptibility testing. This study aimed to modify Muller-Hinton agar (MHA) to improve colistin diffusion in agar.
methodsMHA was modified by reducing the agar concentration from 100% to 30% and supplementing with protamine. We tested 60 gram-negative clinical isolates of
resultsThe modified MHA yielded the best performance metrics, including 94.7% sensitivity, 100% specificity, and an area under the curve of 0.995 (95% confidence interval, 0.982-1.000),
conclusionsA reduction of the agar concentration from 100% to 30% and the addition of protamine improved colistin diffusion in agar and allowed routine colistin susceptibility testing in a clinical microbiology laboratory, but should be handled with caution.
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