ArticleScientific reports2020
Identification and validation of reference genes for RT-qPCR normalization in wheat meiosis.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 34 citations in OpenAlex.
- Validated reference genes for normalization of RT-qPCR in developing organs of wheat to study developmentally/spatio-temporally expressed family genes.Scientific reports · 2025Article
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- Foliar application of plant-derived peptides decreases the severity of leaf rust (Puccinia triticina) infection in bread wheat (Triticum aestivum L.).Journal, genetic engineering & biotechnology · 2024Article
- Transcriptional and protein structural characterization of homogentisate phytyltransferase genes in barley, wheat, and oat.BMC plant biology · 2023Article
- Identification and validation of reference genes in vetiver (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Identification of reference genes for expression studies in the liver and spleen of laying hens housed in cage and cage-free systems.Open veterinary journal · 2023Article
- Selection of Novel Reference Genes by RNA-Seq and Their Evaluation for Normalising Real-Time qPCR Expression Data of Anthocyanin-Related Genes in Lettuce and Wild Relatives.International journal of molecular sciences · 2023Article
- Diversity of transgene integration and gene-editing events in wheat (Frontiers in genome editing · 2023Article
- Overcoming roadblocks forFrontiers in plant science · 2023Review
- Gene Expression Analysis by Quantitative Real-Time PCR for Floral Tissues.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The Landscape of Autophagy-Related (ATG) Genes and Functional Characterization ofInternational journal of molecular sciences · 2022Article
- Assessing equivalent and inverse change in genes between diverse experiments.Frontiers in bioinformatics · 2022Article
- Genome-Wide Expression Analysis of Glyoxalase I Genes Under Hyperosmotic Stress and Existence of a Stress-Responsive Mitochondrial Glyoxalase I Activity in Durum Wheat (Frontiers in plant science · 2022Article
- Selection and Validation of Reference Genes for RT-qPCR Analysis inInternational journal of molecular sciences · 2021Article
- Identification ofInternational journal of molecular sciences · 2021Article
- Selection and validation of appropriate reference genes for RT-qPCR analysis of flowering stages and different genotypes of Iris germanica L.Scientific reports · 2021Article
- Decoding the wheat awn transcriptome and overexpressing TaRca1β in rice for heat stress tolerance.Plant molecular biology · 2021Article
- Redistribution of Meiotic Crossovers Along Wheat Chromosomes by Virus-Induced Gene Silencing.Frontiers in plant science · 2020Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Meiosis is a specialized type of cell division occurring in sexually reproducing organisms to generate haploid cells known as gametes. In flowering plants, male gametes are produced in anthers, being encased in pollen grains. Understanding the genetic regulation of meiosis key events such as chromosome recognition and pairing, synapsis and recombination, is needed to manipulate chromosome associations for breeding purposes, particularly in important cereal crops like wheat. Reverse transcription-quantitative PCR (RT-qPCR) is widely used to analyse gene expression and to validate the results obtained by other transcriptomic analyses, like RNA-seq. Selection and validation of appropriate reference genes for RT-qPCR normalization is essential to obtain reproducible and accurate expression data. In this work, twelve candidate reference genes were evaluated using the mainstream algorithms geNorm, Normfinder, BestKeeper and ΔCt, then ranked from most to least suitable for normalization with RefFinder. Different sets of reference genes were recommended to normalize gene expression data in anther meiosis of bread and durum wheat, their corresponding genotypes in the absence of the Ph1 locus and for comparative studies among wheat genotypes. Comparisons between meiotic (anthers) and somatic (leaves and roots) wheat tissues were also carried out. To the best of our knowledge, our study provides the first comprehensive list of reference genes for robust RT-qPCR normalization to study differentially expressed genes during male meiosis in wheat in a breeding framework.
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