ArticleScientific reports2020
Evoked and spontaneous pain assessment during tooth pulp injury.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- D1 and D2 neurons in medial nucleus accumbens participate in chronic orofacial pain phenotypes.Pain reports · 2026Article
- A mouse model of classical trigeminal neuralgia via intradural compression of the trigeminal nerve.The journal of headache and pain · 2025Article
- Tooth Pulp Afferents and Transient Receptor Potential (TRP) Ion Channels as Key Regulators of Pulp Homeostasis, Inflammation, and Pain.International journal of molecular sciences · 2025Review
- Directed Self-Assembly of Magnetic Bioceramic Deep Inside Dentinal Tubules May Alleviate Dental Hypersensitivity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Mechanically Induced Pulpitis: A Rat Model That Preserves Animal Well-Being.Biomedicines · 2025Article
- The grimace scale: a useful tool for assessing pain in laboratory animals.Experimental animals · 2024Review
- An automaton for preclinical pain testing.Cell reports methods · 2023Article
- Transcriptional profiling of dental sensory and proprioceptive trigeminal neurons using single-cell RNA sequencing.International journal of oral science · 2023Article
- Assessing Orofacial Pain Behaviors in Animal Models: A Review.Brain sciences · 2023Review
- Electroacupuncture exerts prolonged analgesic and neuroprotective effects in a persistent dental pain model induced by multiple dental pulp injuries: GABAergic interneurons-astrocytes interaction.Frontiers in immunology · 2023Article
- Rodent models of postherpetic neuralgia: How far have we reached?Frontiers in immunology · 2023Review
- Activation of the P2X7 receptor in the dental pulp tissue contributes to the pain in rats with acute pulpitis.Molecular pain · 2022Article
- Measuring Mouse Somatosensory Reflexive Behaviors with High-speed Videography, Statistical Modeling, and Machine Learning.Neuromethods · 2022Article
- Vital Pulp Therapy in Permanent Teeth with Irreversible Pulpitis Caused by Caries: A Prospective Cohort Study.Journal of personalized medicine · 2021Article
- Upregulation of Toll-like Receptor 2 in Dental Primary Afferents Following Pulp Injury.Experimental neurobiology · 2021Article
- Methods Used and Application of the Mouse Grimace Scale in Biomedical Research 10 Years on: A Scoping Review.Animals : an open access journal from MDPI · 2021Article
- Grimace Scores: Tools to Support the Identification of Pain in Mammals Used in Research.Animals : an open access journal from MDPI · 2020Review
- Analgesic and Neuroprotective Effects of Electroacupuncture in a Dental Pulp Injury Model-A Basic Research.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Injury of the tooth pulp is excruciatingly painful and yet the receptors and neural circuit mechanisms that transmit this form of pain remain poorly defined in both the clinic and preclinical rodent models. Easily quantifiable behavioral assessment in the mouse orofacial area remains a major bottleneck in uncovering molecular mechanisms that govern inflammatory pain in the tooth. In this study we sought to address this problem using the Mouse Grimace Scale and a novel approach to the application of mechanical Von Frey hair stimuli. We use a dental pulp injury model that exposes the pulp to the outside environment, a procedure we have previously shown produces inflammation. Using RNAscope technology, we demonstrate an upregulation of genes that contribute to the pain state in the trigeminal ganglia of injured mice. We found that mice with dental pulp injury have greater Mouse Grimace Scores than sham within 24 hours of injury, suggestive of spontaneous pain. We developed a scoring system of mouse refusal to determine thresholds for mechanical stimulation of the face with Von Frey filaments. This method revealed that mice with a unilateral dental injury develop bilateral mechanical allodynia that is delayed relative to the onset of spontaneous pain. This work demonstrates that tooth pain can be quantified in freely behaving mice using approaches common for other types of pain assessment. Harnessing these assays in the orofacial area during gene manipulation should assist in uncovering mechanisms for tooth pulp inflammatory pain and other forms of trigeminal pain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.