ArticleScientific reports2020
Real-time cholesterol sorting in Plasmodium falciparum-erythrocytes as revealed by 3D label-free imaging.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Imaging malaria parasites across scales and time.Journal of microscopy · 2026Review
- Dynamics of detergent-resistant membranes of Plasmodium falciparum during blood stage development.Scientific reports · 2025Article
- Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization.European journal of cell biology · 2025Article
- Harnessing cholesterol uptake of malaria parasites for therapeutic applications.EMBO molecular medicine · 2024Article
- Chemogenomic Profiling of a Plasmodium falciparum Transposon Mutant Library Reveals Shared Effects of Dihydroartemisinin and Bortezomib on Lipid Metabolism and Exported Proteins.Microbiology spectrum · 2023Article
- Lack of Nck1 protein and Nck-CD3 interaction caused the increment of lipid content in Jurkat T cells.BMC molecular and cell biology · 2022Article
- The flexibility of Apicomplexa parasites in lipid metabolism.PLoS pathogens · 2022Review
- Dramatic Consequences of Reducing Erythrocyte Membrane Cholesterol on Plasmodium falciparum.Microbiology spectrum · 2022Article
- Budding pouches and associated bubbles: 3D visualization of exo-membrane structures inFrontiers in cellular and infection microbiology · 2022Article
- The role of cholesterol in invasion and growth of malaria parasites.Frontiers in cellular and infection microbiology · 2022Review
- Comparing infrared spectroscopic methods for the characterization of Plasmodium falciparum-infected human erythrocytes.Communications chemistry · 2021Article
- Ezetimibe blocksParasitology · 2021Article
- Breakdown in membrane asymmetry regulation leads to monocyte recognition of P. falciparum-infected red blood cells.PLoS pathogens · 2021Article
- Malaria Parasites Hijack Host Receptors From Exosomes to Capture Lipoproteins.Frontiers in cell and developmental biology · 2021Article
- Depletion of cholesterol could be associated with modulation of progesterone but not other sex hormone levels during Plasmodium falciparum infection in humans: a cross-sectional study from Zaria, Nigeria.Parasitology research · 2020Article
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4 authors.
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Abstract
Cholesterol, a necessary component of animal cell membranes, is also needed by the lethal human malaria parasite Plasmodium falciparum. Because P. falciparum lacks a cholesterol synthesis pathway and malaria patients have low blood cholesterol, we speculated that it scavenges cholesterol from them in some way. We used time-lapse holotomographic microscopy to observe cholesterol transport in live P. falciparum parasites and structurally investigate erythrocyte membranes, both during and after P. falciparum invasion of human erythrocytes. After P. falciparum initially acquired free cholesterol or inner erythrocytic membrane-derived cholesterol, we observed budding lipid membranes elongating into the cytosol and/or membrane segments migrating there and eventually fusing with the parasite membranes, presumably at the parasitophorous vacuole membrane (PVM). Finally, the cholesterol-containing segments were seen to surround the parasite nucleus. Our imaging data suggest that a novel membrane transport system operates in the cytosol of P. falciparum-infected erythrocytes as a cholesterol import system, likely between the PVM and the erythrocyte membrane, and that this transportation process occurs during the live erythrocyte stages of P. falciparum.
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