ArticleScientific reports2020
Interactions of graphene oxide and graphene nanoplatelets with the in vitro Caco-2/HT29 model of intestinal barrier.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Investigating the Therapeutic Potential of Tamarix aphylla Leaf Extract Against Toxicity Caused by Graphene Nanosheets in Cirrhinus mrigala.Veterinary medicine and science · 2026Article
- Toxic or non-toxic: a critical assessment of the contradictory results for selected graphene family materials.Frontiers in toxicology · 2026Review
- Exploring 2D Graphene-Based Nanomaterials for Biomedical Applications: A Theoretical Modeling Perspective.Small science · 2025Article
- Toxicity Mechanisms of Microplastic and Its Effects on Ruminant Production: A Review.Biomolecules · 2025Review
- Enhancement of 5-Fluorouracil Drug Delivery in a Graphene Oxide Containing Electrospun Chitosan/Polyvinylpyrrolidone Construct.Materials (Basel, Switzerland) · 2024Article
- Environmental and Health Impacts of Graphene and Other Two-Dimensional Materials: A Graphene Flagship Perspective.ACS nano · 2024Review
- Human and environmental impacts of nanoparticles: a scoping review of the current literature.BMC public health · 2023Article
- Measuring DNA modifications with the comet assay: a compendium of protocols.Nature protocols · 2023Review
- Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected withJournal of inflammation research · 2023Article
- Hazard Assessment of the Effects of Acute and Chronic Exposure to Permethrin, Copper Hydroxide, Acephate, and Validamycin Nanopesticides on the Physiology ofInternational journal of molecular sciences · 2022Article
- Immunotoxicity of Carbon-Based Nanomaterials, Starring Phagocytes.International journal of molecular sciences · 2022Review
- Genotoxicity of Graphene-Based Materials.Nanomaterials (Basel, Switzerland) · 2022Review
- Biomimetically Inspired Highly Homogeneous Hydrophilization of Graphene with Poly(l-DOPA): Toward Electroconductive Coatings from Water-Processable Paints.ACS sustainable chemistry & engineering · 2022Article
- Article
- Direct and Indirect Genotoxicity of Graphene Family Nanomaterials on DNA-A Review.Nanomaterials (Basel, Switzerland) · 2021Review
- Ex Vivo Human Colon Tissue Exposure to Pristine Graphene Activates Genes Involved in the Binding, Adhesion and Proliferation of Epithelial Cells.International journal of molecular sciences · 2021Article
- IL-6/STAT3 signaling pathway regulates the proliferation and damage of intestinal epithelial cells in patients with ulcerative colitis via H3K27ac.Experimental and therapeutic medicine · 2021Article
- Genotoxicity of Nanomaterials: Advanced In Vitro Models and High Throughput Methods for Human Hazard Assessment-A Review.Nanomaterials (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbon-based nanomaterials are being increasingly used, demanding strong information to support their safety in terms of human health. As ingestion is one of the most important exposure routes in humans, we have determined their potential risk by using an in vitro model simulating the human intestinal barrier and evaluated the effects of both graphene oxide (GO) and graphene nanoplatelets (GNPs). A coculture of differentiated Caco-2/HT29 cells presenting inherent intestinal epithelium characteristics (i.e. mucus secretion, brush border, tight junctions, etc.) were treated with GO or GNPs for 24 h. Different endpoints such as viability, membrane integrity, NPs localization, cytokines secretion, and genotoxic damage were evaluated to have a wide view of their potentially harmful effects. No cytotoxic effects were observed in the cells that constitute the barrier model. In the same way, no adverse effects were detected neither in the integrity of the barrier (TEER) nor in its permeability (LY). Nevertheless, a different bio-adhesion and biodistribution behavior was observed for GO and GNPs by confocal microscopy analysis, with a more relevant uptake of GNPs. No oxidative damage induction was detected, either by the DCFH-DA assay or the FPG enzyme in the comet assay. Conversely, both GO and GNPs were able to induce DNA breaks, as observed in the comet assay. Finally, low levels of anti-inflammatory cytokines were detected, suggesting a weak anti-inflammatory response. Our results show the moderate/severe risk posed by GO/GNPs exposures, given the observed genotoxic effects, suggesting that more extensive genotoxic evaluations must be done to properly assess the genotoxic hazard of these nanomaterials.
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