ArticleParasites & vectors2020
Blood-meal analysis of Culicoides (Diptera: Ceratopogonidae) reveals a broad host range and new species records for Romania.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Culicoides biting midges (Diptera: Ceratopogonidae) feeding on donkeys in the United Kingdom, with reference to the risk of transmission and persistence of African horse sickness virus.Medical and veterinary entomology · 2026Article
- Diversity of Dipteran Vectors Associated with Different Mammalian Hosts in a Peri-Urban Zoological Garden in NW Spain.Acta parasitologica · 2026Article
- First Evidence of Bovine Leukemia Virus in Culicoides Biting Midges and the Stable Fly Stomoxys calcitrans Collected on Cattle Farms in Poland.Transboundary and emerging diseases · 2026Article
- Diversity and abundance of Culicoides on goat and cattle farms in the southern part of the Republic of Korea.Parasite (Paris, France) · 2026Article
- Detection of avian haemosporidian parasite DNA from potential vector arthropods distributed in the endangered bird conservation facility at Sado Island of Japan.The Journal of veterinary medical science · 2025Article
- Investigating the role of stable flies (Stomoxys calcitrans) and biting midges of the genus Culicoides as potential mechanical vectors of African swine fever virus in Lithuania, Poland and Romania.Parasites & vectors · 2025Article
- Determinants of vector-borne avian pathogen occurrence in a mosaic of habitat fragmentation in California.Parasites & vectors · 2025Article
- Host blood meal analysis of Culicoides oxystoma (Diptera: Ceratopogonidae) in Tunisia.Parasitology research · 2025Article
- Molecular detection of parasites and host preference in wild-caught Culicoides biting midges (Diptera: Ceratopogonidae) in Chiang Mai and Nakhon Si Thammarat Provinces, Thailand.Parasite (Paris, France) · 2025Article
- High Abundance ofInsects · 2024Article
- Bluetongue Virus Infection in Farm Dogs Exposed to an Infected Sheep Flock in South Africa.Transboundary and emerging diseases · 2024Article
- Molecular Identification ofInsects · 2023Article
- Effects of storage conditions and digestion time on DNA amplification of biting midge (Culicoides) blood meals.Parasites & vectors · 2023Article
- Viral diversity and blood-feeding patterns of AfrotropicalFrontiers in microbiology · 2023Article
- Article
- Culicoides species community composition and feeding preferences in two aquatic ecosystems in northern Spain.Parasites & vectors · 2022Article
- First Report of Culicoides Biting Midges (Diptera: Ceratopogonidae) Attacking People in Italy, With the Description of Extreme Larval Breeding Sites and Diurnal Activity of Culicoides riethi.Journal of medical entomology · 2022Article
- Microsporidia, a Highly Adaptive Organism and Its Host Expansion to Humans.Frontiers in cellular and infection microbiology · 2022Review
- Profiling of RNA Viruses in Biting Midges (mSphere · 2021Article
- Validation of an Effective Protocol forInsects · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCulicoides biting midges are potential vectors of different pathogens. However, especially for eastern Europe, there is a lack of knowledge on the host-feeding patterns of this vector group. Therefore, this study aimed to identify Culicoides spp. and their vertebrate hosts collected in a wetland ecosystem.
methodsCulicoides spp. were collected weekly from May to August 2017, using Biogents traps with UV light at four sites in the Danube Delta Biosphere Reserve, Romania. Vectors and hosts were identified with a DNA barcoding approach. The mitochondrial cytochrome c oxidase subunit 1 was used to identify Culicoides spp., while vertebrate hosts were determined targeting cytochrome b or 16S rRNA gene fragments. A maximum likelihood phylogenetic tree was constructed to verify the biting midge identity against other conspecific Palaearctic Culicoides species. A set of unfed midges was used for morphological confirmation of species identification using slide-mounted wings.
resultsBarcoding allowed the species identification and detection of corresponding hosts for 1040 (82.3%) of the 1264 analysed specimens. Eight Culicoides spp. were identified with Culicoides griseidorsum, Culicoides puncticollis and Culicoides submaritimus as new species records for Romania. For 39 specimens no similar sequences were found in GenBank. This group of unknown Culicoides showed a divergence of 15.6-16.3% from the closest identified species and clustered in a monophyletic clade, i.e. a novel species or a species without reference sequences in molecular libraries. For all Culicoides spp., nine mammalian and 24 avian species were detected as hosts. With the exception of C. riethi (n = 12), at least one avian host was detected for all Culicoides spp., but this host group only dominated for Culicoides kibunensis and the unknown Culicoides sp.. The most common host group were mammals (n = 993, 87.6% of all identified blood sources) dominated by cattle (n = 817, 70.6%).
conclusionsMost Culicoides spp. showed a broad host-feeding pattern making them potential bridge vectors. At the same time, new records of biting midge species for Romania, as well as a potentially unknown Culicoides species, highlight the lack of knowledge regarding the biting midge species and their genetic diversity in eastern Europe.
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