Evidence map›Paper›PMID 32065636›Full record

ArticlePlant & cell physiology2020

Downregulation of a Mitochondrial NAD+ Transporter (NDT2) Alters Seed Production and Germination in Arabidopsis.

Elias Feitosa-Araujo, Izabel de Souza Chaves, Alexandra Florian, Paula da Fonseca-Pereira, Jorge Alberto Condori Apfata, Elmien Heyneke, David Barbosa Medeiros, Marcel Viana Pires, Tabea Mettler-Altmann, H Ekkehard Neuhaus and 7 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant & cell physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
4.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Review
  9. Review
  10. Conserved and Opposite Transcriptome Patterns during Germination inInternational journal of molecular sciences · 2020
    Article
  11. In Vivo NADH/NADThe Plant cell · 2020
    Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 5 institutions in 3 countries.

Elias Feitosa-AraujoMax Planck Partner Group, Departamento de Biologia Vegetal, Universidade Federal de Vi�osa, Vi�osa 36570-900, Minas Gerais, Brazil.
Izabel de Souza ChavesMax Planck Partner Group, Departamento de Biologia Vegetal, Universidade Federal de Vi�osa, Vi�osa 36570-900, Minas Gerais, Brazil.
Alexandra FlorianMax-Planck-Institute of Molecular Plant Physiology, Am M�hlenberg 1, Potsdam-Golm 14476, Germany.
Paula da Fonseca-PereiraMax Planck Partner Group, Departamento de Biologia Vegetal, Universidade Federal de Vi�osa, Vi�osa 36570-900, Minas Gerais, Brazil.
Jorge Alberto Condori ApfataMax Planck Partner Group, Departamento de Biologia Vegetal, Universidade Federal de Vi�osa, Vi�osa 36570-900, Minas Gerais, Brazil.
Elmien HeynekeMax-Planck-Institute of Molecular Plant Physiology, Am M�hlenberg 1, Potsdam-Golm 14476, Germany.
David Barbosa MedeirosMax Planck Partner Group, Departamento de Biologia Vegetal, Universidade Federal de Vi�osa, Vi�osa 36570-900, Minas Gerais, Brazil.
Marcel Viana PiresMax Planck Partner Group, Departamento de Biologia Vegetal, Universidade Federal de Vi�osa, Vi�osa 36570-900, Minas Gerais, Brazil.
Tabea Mettler-AltmannDepartment of Plant Biochemistry, Heinrich Heine University D�sseldorf, D�sseldorf 40225, Germany.
H Ekkehard NeuhausDepartment of Plant Physiology, University of Kaiserslautern, Kaiserslautern 67663, Germany.
Ferdinando PalmieriDepartment of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, Bari 70125, Italy.
Wagner L Araï JoMax Planck Partner Group, Departamento de Biologia Vegetal, Universidade Federal de Vi�osa, Vi�osa 36570-900, Minas Gerais, Brazil.
Toshihiro ObataMax-Planck-Institute of Molecular Plant Physiology, Am M�hlenberg 1, Potsdam-Golm 14476, Germany.
Andreas P M WeberDepartment of Plant Biochemistry, Heinrich Heine University D�sseldorf, D�sseldorf 40225, Germany.
Nicole LinkaDepartment of Plant Biochemistry, Heinrich Heine University D�sseldorf, D�sseldorf 40225, Germany.
Alisdair R FernieMax-Planck-Institute of Molecular Plant Physiology, Am M�hlenberg 1, Potsdam-Golm 14476, Germany.
Adriano Nunes-NesiMax Planck Partner Group, Departamento de Biologia Vegetal, Universidade Federal de Vi�osa, Vi�osa 36570-900, Minas Gerais, Brazil.
Universidade Federal de Viçosa · BRMax Planck Institute of Molecular Plant Physiology · DEHeinrich Heine University Düsseldorf · DEUniversity of Bari Aldo Moro · ITUniversity of Kaiserslautern · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite the fundamental importance of nicotinamide adenine dinucleotide (NAD+) for metabolism, the physiological roles of NAD+ carriers in plants remain unclear. We previously characterized the Arabidopsis thaliana gene (At1g25380), named AtNDT2, encoding a protein located in the mitochondrial inner membrane, which imports NAD+ from the cytosol using ADP and AMP as counter-exchange substrates for NAD+. Here, we further investigated the physiological roles of NDT2, by isolating a T-DNA insertion line, generating an antisense line and characterizing these genotypes in detail. Reduced NDT2 expression affected reproductive phase by reducing total seed yield. In addition, reduced seed germination and retardation in seedling establishment were observed in the mutant lines. Moreover, remarkable changes in primary metabolism were observed in dry and germinated seeds and an increase in fatty acid levels was verified during seedling establishment. Furthermore, flowers and seedlings of NDT2 mutants displayed upregulation of de novo and salvage pathway genes encoding NAD+ biosynthesis enzymes, demonstrating the transcriptional control mediated by NDT2 activity over these genes. Taken together, our results suggest that NDT2 expression is fundamental for maintaining NAD+ balance amongst organelles that modulate metabolism, physiology and developmental processes of heterotrophic tissues.

Indexed as

Gene Expression Regulation, PlantArabidopsis ProteinsBiological TransportDown-RegulationFlowersGenotypeGerminationHeterotrophic ProcessesMitochondriaMitochondrial ProteinsNADNucleotidesNucleotide Transport ProteinsPyridinesReproductionSeedsArabidopsis ProteinsAt1g25380 protein, ArabidopsisMitochondrial ProteinsNADNucleotidesNucleotide Transport ProteinspyridinePyridinesMetabolite transportMitochondriaNicotinamide adenine dinucleotideSeed germinationSeed production

Identifiers

PMID32065636
PMCPMC7217668
OpenAlexW3007396907

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.