ArticleFrontiers in plant science2019
Arabidopsis Seed Stored mRNAs are Degraded Constantly over Aging Time, as Revealed by New Quantification Methods.
Article in Frontiers in plant science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Aging and longevity in decades-old genebanked seeds from U.S. endangered plant species: Assessments using survival and RNA integrity assays.American journal of botany · 2026Article
- RNA-Binding Protein OsGRP3 Positively Regulates Rice Storability.Plants (Basel, Switzerland) · 2026Article
- Accumulating Heterozygous Deleterious Mutations in Conserved Soybean Germplasm over Successive Regenerations.Plants (Basel, Switzerland) · 2025Article
- Long-term preserved bean seeds exhibit high RNA integrity and high germination potential.Scientific reports · 2025Article
- Assessing the RNA integrity in dry seeds collected from diverse endangered species native to the USA.Frontiers in plant science · 2025Article
- Seed longevity and genome damage.Bioscience reports · 2024Review
- WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition.The Biochemical journal · 2023Article
- Nitric Oxide in Seed Biology.International journal of molecular sciences · 2022Review
- Comparative Seeds Storage Transcriptome Analysis ofInternational journal of molecular sciences · 2022Article
- Identification of the Potential Genes Regulating Seed Germination Speed in Maize.Plants (Basel, Switzerland) · 2022Article
- Exploring Breakthroughs in Three Traits Belonging to Seed Life.Plants (Basel, Switzerland) · 2022Review
- Nitric oxide-an antidote to seed aging modifiesFrontiers in plant science · 2022Article
- Nitric Oxide as a Remedy against Oxidative Damages in Apple Seeds Undergoing Accelerated Ageing.Antioxidants (Basel, Switzerland) · 2021Article
- Barley Seeds miRNome Stability during Long-Term Storage and Aging.International journal of molecular sciences · 2021Article
- Genome-Wide Association Mapping Unravels the Genetic Control of Seed Vigor under Low-Temperature Conditions in Rapeseed (Plants (Basel, Switzerland) · 2021Article
- Analysis of Stored mRNA Degradation in Acceleratedly Aged Seeds of Wheat and Canola in Comparison to Arabidopsis.Plants (Basel, Switzerland) · 2020Article
- The seed water content as a time-independent physiological trait during germination in wild tree species such as Ceiba aesculifolia.Scientific reports · 2020Article
- Lost in Translation: Physiological Roles of Stored mRNAs in Seed Germination.Plants (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How plant seeds age remains poorly understood and effective tools for monitoring seed aging are lacking. Dry seeds contain various stored mRNAs which are believed to be required for protein synthesis during early stages of seed germination. We reasoned that seed stored mRNAs would undergo degradation during seed aging, based on the propensity of mRNAs to degrade. We performed RT-PCR and qPCR analyses to study the changes in stored mRNA levels of Arabidopsis seeds during aging. All stored mRNAs analyzed were gradually degraded in both naturally and artificially aged seeds. The difference in Ct values between aged and control seeds (ΔCt value) was highly correlated with the mRNA fragment size and seed aging time. We derived mathematical equations for estimating the relative amount of undamaged stored mRNAs and frequency of the breakdown at one nucleotide level for individual mRNAs. Stored mRNAs were found to break down randomly. The frequency of breaks per nucleotide per day, which we named
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