ReviewBrain structure & function2020
Neuroanatomical tract-tracing techniques that did go viral.
Review in Brain structure & function, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed.
- Viral Tracing Gone Viral: Expanded Possibilities Call for Increased Caution in Describing Cerebellar Network Connections.Cerebellum (London, England) · 2026Review
- Structural and functional anatomy of the superior longitudinal fasciculus: a historical and contemporary narrative review.Anatomical science international · 2026Review
- The Anatomical Connections of Nucleus Accumbens with Brainstem and Cerebellum in the Rat and Human Subjects.Cerebellum (London, England) · 2026Article
- Developmental human brain connectome from fetal stage to early childhood.Developmental cognitive neuroscience · 2026Review
- Probabilistic spike propagation shapes sympathetic output in mouse preganglionic neurons.bioRxiv : the preprint server for biology · 2026Article
- Spatial organization and sex differences of paraventricular hypothalamic oxytocin neurons projecting to distinct brain regions.Brain structure & function · 2026Article
- What needs to be standardized for reliable, reproducible, and robust tractography?GigaScience · 2026Review
- Visualization of Selective Neural Pathways by Double Viral Infection Method.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Optogenetic Approach for In Vivo Manipulation of Neural Circuits Using Lentiviral Vectors.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Historical trends in neuroanatomical tract-tracing techniques.Anatomical science international · 2025Review
- Hippocampal architecture viewed through the eyes of methodological development.Anatomical science international · 2025Review
- Lateral Thinking: Exploring the Lateral Entorhinal Cortex Projections to the Hippocampus in the Rodent Brain.The Journal of comparative neurology · 2025Review
- Neurotransmitters' white matter mapping unveils the neurochemical fingerprints of stroke.Nature communications · 2025Article
- Structural MRI of brain similarity networks.Nature reviews. Neuroscience · 2025Review
- Software and pipelines for registration and analyses of rodent brain image data in reference atlas space.Frontiers in neuroinformatics · 2025Article
- Spinal cord injury modeling: from modeling to evaluation using rats as examples.Frontiers in neurology · 2025Review
- Afferent innervation of the kidney: projections to and processing in the central nervous system.Frontiers in physiology · 2025Review
- Spatially integrated cortico-subcortical tracing data for analyses of rodent brain topographical organization.Scientific data · 2024Article
- Translation of monosynaptic circuits underlying amygdala fMRI neurofeedback training.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2024Article
- Tractometry of Human Visual White Matter Pathways in Health and Disease.Magnetic resonance in medical sciences : MRMS : an official journal of Japan Society of Magnetic Resonance in Medicine · 2024Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Neuroanatomical tracing methods remain fundamental for elucidating the complexity of brain circuits. During the past decades, the technical arsenal at our disposal has been greatly enriched, with a steady supply of fresh arrivals. This paper provides a landscape view of classical and modern tools for tract-tracing purposes. Focus is placed on methods that have gone viral, i.e., became most widespread used and fully reliable. To keep an historical perspective, we start by reviewing one-dimensional, standalone transport-tracing tools; these including today's two most favorite anterograde neuroanatomical tracers such as Phaseolus vulgaris-leucoagglutinin and biotinylated dextran amine. Next, emphasis is placed on several classical tools widely used for retrograde neuroanatomical tracing purposes, where Fluoro-Gold in our opinion represents the best example. Furthermore, it is worth noting that multi-dimensional paradigms can be designed by combining different tracers or by applying a given tracer together with detecting one or more neurochemical substances, as illustrated here with several examples. Finally, it is without any doubt that we are currently witnessing the unstoppable and spectacular rise of modern molecular-genetic techniques based on the use of modified viruses as delivery vehicles for genetic material, therefore, pushing the tract-tracing field forward into a new era. In summary, here, we aim to provide neuroscientists with the advice and background required when facing a choice on which neuroanatomical tracer-or combination thereof-might be best suited for addressing a given experimental design.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.