ArticleAntonie van Leeuwenhoek2020
Genome rearrangements and megaplasmid loss in the filamentous bacterium Kitasatospora viridifaciens are associated with protoplast formation and regeneration.
Article in Antonie van Leeuwenhoek, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- Essential and dispensable domains of DivIVA for walled growth in filamentous Actinomycetota.Open biology · 2025Article
- The parABSm system is involved in megaplasmid partitioning and genome integrity maintenance in Thermus thermophilus.G3 (Bethesda, Md.) · 2023Article
- Strain Improvement and Strain Maintenance Revisited. The Use ofAntibiotics (Basel, Switzerland) · 2021Article
- Genome editing reveals that pSCL4 is required for chromosome linearity inMicrobial genomics · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Filamentous Actinobacteria are multicellular bacteria with linear replicons. Kitasatospora viridifaciens DSM 40239 contains a linear 7.8 Mb chromosome and an autonomously replicating plasmid KVP1 of 1.7 Mb. Here we show that lysozyme-induced protoplast formation of the multinucleated mycelium of K. viridifaciens drives morphological diversity. Characterisation and sequencing of an individual revertant colony that had lost the ability to differentiate revealed that the strain had not only lost most of KVP1 but also carried deletions in the right arm of the chromosome. Strikingly, the deletion sites were preceded by insertion sequence elements, suggesting that the rearrangements may have been caused by replicative transposition and homologous recombination between both replicons. These data indicate that protoplast formation is a stressful process that can lead to profound genetic changes.
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