Evidence map›Paper›PMID 32060572›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2020

Genetic and transcriptional dissection of resistance to Claviceps purpurea in the durum wheat cultivar Greenshank.

Anna Gordon, Curt McCartney, Ron E Knox, Nelzo Ereful, Colin W Hiebert, David J Konkin, Ya-Chih Hsueh, Vijai Bhadauria, Mara Sgroi, Donal M O'Sullivan and 3 more

Open access · hybridAbstract read
In one paragraph

Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
7.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 28 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 2 countries.

Anna GordonNIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK. anna.gordon@niab.com.ORCID http://orcid.org/0000-0003-3837-0237
Curt McCartneyMorden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB, R6M 1Y5, Canada.
Ron E KnoxSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.
Nelzo ErefulNIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK.
Colin W HiebertMorden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB, R6M 1Y5, Canada.
David J KonkinAquatic and Crop Resource Development, National Research Council Canada, Saskatoon, SK, Canada.
Ya-Chih HsuehAquatic and Crop Resource Development, National Research Council Canada, Saskatoon, SK, Canada.
Vijai BhadauriaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.
Mara SgroiDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge, CB2 3EA, UK.
Donal M O'SullivanSchool of Agriculture, Policy and Development, University of Reading, Whiteknights, Reading, RG6 6AR, UK.
Caroline HadleySchool of Agriculture, Policy and Development, University of Reading, Whiteknights, Reading, RG6 6AR, UK.
Lesley A BoydNIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK.
Jim G MenziesMorden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB, R6M 1Y5, Canada. jim.menzies@canada.ca.ORCID http://orcid.org/0000-0002-5591-8957
Agriculture and Agri-Food Canada · CANational Institute of Agricultural Botany · GBSaskatchewan Research Council (Canada) · CAUniversity of Reading · GBUniversity of Cambridge · GB

Funding

Biotechnology and Biological Sciences Research Council BB/G020418/1
6 · The paper itself

Abstract

key messageFour QTL for ergot resistance (causal pathogen Claviceps purpurea) have been identified in the durum wheat cultivar Greenshank. Claviceps purpurea is a pathogen of grasses that infects flowers, replacing the seed with an ergot sclerotium. Ergot presents a significant problem to rye, barley and wheat, in particular hybrid seed production systems. In addition, there is evidence that the highly toxic alkaloids that accumulate within sclerotia can cross-contaminate otherwise healthy grain. Host resistance to C. purpurea is rare, few resistance loci having been identified. In this study, four ergot resistance loci are located on chromosomes 1B, 2A, 5A and 5B in the durum wheat cv. Greenshank. Ergot resistance was assessed through analysis of phenotypes associated with C. purpurea infection, namely the number of inoculated flowers that produced sclerotia, or resulted in ovary death but no sclerotia, the levels of honeydew produced, total sclerotia weight and average sclerotia weight and size per spike. Ergot testing was undertaken in Canada and the UK. A major effect QTL, QCp.aafc.DH-2A, was detected in both the Canadian and UK experiments and had a significant effect on honeydew production levels. QCp.aafc.DH-5B had the biggest influence on total sclerotia weight per spike. QCp.aafc.DH-1B was only detected in the Canadian experiments and QCp.aafc.DH-5A in the UK experiment. An RNASeq analysis, undertaken to identify wheat differentially expressed genes associated with different combinations of the four ergot resistance QTL, revealed a disproportionate number of DEGs locating to the QCp.aafc.DH-1B, QCp.aafc.DH-2A and QCp.aafc.DH-5B QTL intervals.

Indexed as

Quantitative Trait LociClavicepsDisease ResistanceGenes, PlantHordeumPhenotypePlant DiseasesPoaceaeTranscription, GeneticTriticum

Identifiers

PMID32060572
PMCPMC7237535
OpenAlexW3006200970

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.