ArticleNature protocols2020
A preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction.
Article in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
69 citing papers in PubMed, 1 synthesis or guideline pooled it, 146 citations in OpenAlex.
- Effect of chitosan/inorganic nanomaterial scaffolds on bone regeneration and related influencing factors in animal models: A systematic review.Frontiers in bioengineering and biotechnology · 2022Pooled it
- A bioprinted periosteum organoid enables functional repair of critical-sized bone defects.Bioactive materials · 2026Article
- Biomass-Derived Hydrogels for Load-Bearing Connective Tissue Repair: Integrative Reinforcement, Bio-Functional Design, and Emerging Pathways Toward Clinical Translation.Advanced healthcare materials · 2026Review
- Microenvironment engineering with injectable hydrogel-based biofunctional scaffolds for augmenting bone defect regeneration.Journal of nanobiotechnology · 2026Review
- Comparative assessment of the reaming characteristics of two different technologies for intramedullary bone graft harvesting.Archives of orthopaedic and trauma surgery · 2026Article
- Efficient derivation of stable sheep embryonic stem cells opens a new avenue for agricultural and biomedical application.Journal of advanced research · 2026Article
- Advancements in bone organoids: perspectives on construction methodologies and application strategies.Journal of advanced research · 2026Review
- Membrane-free bone regeneration using biphasic calcium phosphate, heterologous fibrin biopolymer, and photobiomodulation: anThe journal of venomous animals and toxins including tropical diseases · 2026Article
- Synergistic integration of biomaterials in orthopedic implantation for infection preventing and tissue engineering.Regenerative biomaterials · 2026Review
- Reconstruction of a segmental bone defect using vascularized tissue-engineered bone substitutes: An animal study.PloS one · 2026Article
- Nanozymes for Bone Regeneration: Mechanistic Insights into Immune and Metabolic Microenvironment Modulation.International journal of nanomedicine · 2026Review
- Surgical induction model of femoral defect in Wistar rats for bone repair histology.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas · 2026Article
- Investigating the foreign body response and regenerative mechanisms in medical-grade polycaprolactone scaffold guided breast reconstruction in a porcine model.Frontiers in bioengineering and biotechnology · 2026Article
- Definition of critical skin defect and concepts of structural and functional repairs: Proposal and verification in a rat model.Animal models and experimental medicine · 2026Article
- Mechanobiologically-optimized non-resorbable artificial bone for patient-matched scaffold-guided bone regeneration.Nature communications · 2025Article
- Additively manufactured biodegradable porous FeMn-akermanite scaffolds for critical-size bone defects: the firstMaterials today. Bio · 2025Article
- A strategy for challenging tumorous bone regeneration by borosilicate bioactive glass boosting moderate magnetic hyperthermia.Nature communications · 2025Article
- Curcumin-encapsulated exosomes in bisphosphonate-modified hydrogel microspheres promote bone repair through macrophage polarization and DNA damage mitigation.Materials today. Bio · 2025Article
- Assessing Cardiopulmonary Safety of Intramedullary Bone Graft Harvesting: A Comparative Study of the RIA 2 System and the ARA Concept.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Fluorine-Incorporated Biogenic Hydroxyapatite Enhances Socket Bone Healing via Addressing Macrophage-Mediated Inflammatory Response.Bioengineering (Basel, Switzerland) · 2025Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Critical-size bone defects, which require large-volume tissue reconstruction, remain a clinical challenge. Bone engineering has the potential to provide new treatment concepts, yet clinical translation requires anatomically and physiologically relevant preclinical models. The ovine critical-size long-bone defect model has been validated in numerous studies as a preclinical tool for evaluating both conventional and novel bone-engineering concepts. With sufficient training and experience in large-animal studies, it is a technically feasible procedure with a high level of reproducibility when appropriate preoperative and postoperative management protocols are followed. The model can be established by following a procedure that includes the following stages: (i) preoperative planning and preparation, (ii) the surgical approach, (iii) postoperative management, and (iv) postmortem analysis. Using this model, full results for peer-reviewed publication can be attained within 2 years. In this protocol, we comprehensively describe how to establish proficiency using the preclinical model for the evaluation of a range of bone defect reconstruction options.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.