ArticleNature communications2020
In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
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- Genetic coupling of enhancer activity and connectivity in gene expression control.Nature communications · 2025Article
- Active enhancers: recent research advances and insights into disease.Biology direct · 2024Review
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- New Drosophila promoter-associated architectural protein Mzfp1 interacts with CP190 and is required for housekeeping gene expression and insulator activity.Nucleic acids research · 2024Article
- Genome organization regulates nuclear pore complex formation and promotes differentiation duringGenes & development · 2024Article
- HiCrayon reveals distinct layers of multi-state 3D chromatin organization.bioRxiv : the preprint server for biology · 2024Article
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- Genome organization regulates nuclear pore complex formation and promotes differentiation during Drosophila oogenesis.bioRxiv : the preprint server for biology · 2023Article
- RNA polymerase II pausing regulates chromatin organization in erythrocytes.Nature structural & molecular biology · 2023Article
- Epigenetic regulation of nuclear processes in fungal plant pathogens.PLoS pathogens · 2023Review
- The Drosophila BEAF insulator protein interacts with the polybromo subunit of the PBAP chromatin remodeling complex.G3 (Bethesda, Md.) · 2022Article
- Mode and Tempo of 3D Genome Evolution in Drosophila.Molecular biology and evolution · 2022Article
- New Mutations in the 5' Region of theJournal of developmental biology · 2022Article
- Nuclear receptor activation shapes spatial genome organization essential for gene expression control: lessons learned from the vitamin D receptor.Nucleic acids research · 2022Article
- The role of insulators and transcription in 3D chromatin organization of flies.Genome research · 2022Article
- The stochastic nature of genome organization and function.Current opinion in genetics & development · 2022Review
- Transcription and splicing dynamics during earlyRNA (New York, N.Y.) · 2022Article
- Chromatin Organization and Function inCells · 2021Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosomes are organized into high-frequency chromatin interaction domains called topologically associating domains (TADs), which are separated from each other by domain boundaries. The molecular mechanisms responsible for TAD formation are not yet fully understood. In Drosophila, it has been proposed that transcription is fundamental for TAD organization while the participation of genetic sequences bound by architectural proteins (APs) remains controversial. Here, we investigate the contribution of domain boundaries to TAD organization and the regulation of gene expression at the Notch gene locus in Drosophila. We find that deletion of domain boundaries results in TAD fusion and long-range topological defects that are accompanied by loss of APs and RNA Pol II chromatin binding as well as defects in transcription. Together, our results provide compelling evidence of the contribution of discrete genetic sequences bound by APs and RNA Pol II in the partition of the genome into TADs and in the regulation of gene expression in Drosophila.
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