ArticleNature communications2020
In vitro self-replication and multicistronic expression of large synthetic genomes.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 92 citations in OpenAlex.
- PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized proteins.Nature communications · 2026Article
- Autonomous biogenesis of all thirty proteins of the Escherichia coli translation machinery.Nature communications · 2025Article
- Reconstituting transcription-translation-coupled DNA replication within complex in vitro biological systems.Nature communications · 2025Article
- Synthetic cells by the numbers.iScience · 2025Review
- Genetically Encoded Control ofACS synthetic biology · 2025Article
- Review
- Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method.Nature communications · 2025Article
- Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a recombinant cell-free system.Nature communications · 2025Article
- Sustainable regeneration of 20 aminoacyl-tRNA synthetases in a reconstituted system toward self-synthesizing artificial systems.Science advances · 2025Article
- Self-growing protocell models in aqueous two-phase system induced by internal DNA replication reaction.Nature communications · 2025Article
- Artificial cells with all-aqueous droplet-in-droplet structures for spatially separated transcription and translation.Nature communications · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Cell-Free Gene Expression in Bioprinted Fluidic Networks.ACS synthetic biology · 2024Article
- Cell-Free Protein Expression by a Reconstituted Transcription-Translation System Energized by Sugar Catabolism.Molecules (Basel, Switzerland) · 2024Article
- Leveraging Active Learning to Establish Efficient In Vitro Transcription and Translation from Bacterial Chromosomal DNA.ACS omega · 2024Article
- A genetic circuit on a single DNA molecule as an autonomous dissipative nanodevice.Nature communications · 2024Article
- C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System.ACS synthetic biology · 2023Article
- On biochemical constructors and synthetic cells.Interface focus · 2023Article
- Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles.ACS synthetic biology · 2023Article
- The Long Road to a Synthetic Self-Replicating Central Dogma.Biochemistry · 2023Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The generation of a chemical system capable of replication and evolution is a key objective of synthetic biology. This could be achieved by in vitro reconstitution of a minimal self-sustaining central dogma consisting of DNA replication, transcription and translation. Here, we present an in vitro translation system, which enables self-encoded replication and expression of large DNA genomes under well-defined, cell-free conditions. In particular, we demonstrate self-replication of a multipartite genome of more than 116 kb encompassing the full set of Escherichia coli translation factors, all three ribosomal RNAs, an energy regeneration system, as well as RNA and DNA polymerases. Parallel to DNA replication, our system enables synthesis of at least 30 encoded translation factors, half of which are expressed in amounts equal to or greater than their respective input levels. Our optimized cell-free expression platform could provide a chassis for the generation of a partially self-replicating in vitro translation system.
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