Evidence map›Paper›PMID 32059767›Full record

ArticleCurrent biology : CB2020

Single-Cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting.

Hongjie Li, Tongchao Li, Felix Horns, Jiefu Li, Qijing Xie, Chuanyun Xu, Bing Wu, Justus M Kebschull, Colleen N McLaughlin, Sai Saroja Kolluru and 6 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current biology : CB, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
7.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 58 citations in OpenAlex.

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  7. An integrated anatomical, functional and evolutionary view of thebioRxiv : the preprint server for biology · 2025
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  15. bioRxiv : the preprint server for biology · 2023
    Article
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  19. Proceedings. Biological sciences · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 2 institutions in 1 country.

Hongjie LiDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: hongjie@stanford.edu.
Tongchao LiDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Felix HornsBiophysics Graduate Program, Stanford University, Stanford, CA 94305, USA; Department of Bioengineering and Department of Applied Physics, Stanford University, Stanford, CA 94305, USA.
Jiefu LiDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Qijing XieDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Neurosciences Graduate Program, Stanford University, Stanford, CA 94305, USA.
Chuanyun XuDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Bing WuDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Justus M KebschullDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Colleen N McLaughlinDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Sai Saroja KolluruDepartment of Bioengineering and Department of Applied Physics, Stanford University, Stanford, CA 94305, USA.
Robert C JonesDepartment of Bioengineering and Department of Applied Physics, Stanford University, Stanford, CA 94305, USA.
David VacekDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Anthony XieDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
David J LuginbuhlDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Stephen R QuakeDepartment of Bioengineering and Department of Applied Physics, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, Stanford, CA 94305, USA. Electronic address: quake@stanford.edu.
Liqun LuoDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: lluo@stanford.edu.
Stanford University · USHoward Hughes Medical Institute · US

Funding

Assembly of the Central Olfactory Networks in DrosophilaR01DC005982 · NIDCD · STANFORD UNIVERSITY · PI LIQUN LUO · 2003 to 2026
$8.1M
Characterizing the assembly and age-related decline of neural circuits in Drosophila by single-cell profilingK99AG062746 · NIA · STANFORD UNIVERSITY · PI LI, HONGJIE · 2019 to 2020
$254k
Howard Hughes Medical InstituteNIA NIH HHS K99 AG062746NIDCD NIH HHS R01 DC005982
6 · The paper itself

Abstract

The regulatory mechanisms by which neurons coordinate their physiology and connectivity are not well understood. The Drosophila olfactory receptor neurons (ORNs) provide an excellent system to investigate this question. Each ORN type expresses a unique olfactory receptor, or a combination thereof, and sends their axons to a stereotyped glomerulus. Using single-cell RNA sequencing, we identified 33 transcriptomic clusters for ORNs and mapped 20 to their glomerular types, demonstrating that transcriptomic clusters correspond well with anatomically and physiologically defined ORN types. Each ORN type expresses hundreds of transcription factors. Transcriptome-instructed genetic analyses revealed that (1) one broadly expressed transcription factor (Acj6) only regulates olfactory receptor expression in one ORN type and only wiring specificity in another type, (2) one type-restricted transcription factor (Forkhead) only regulates receptor expression, and (3) another type-restricted transcription factor (Unplugged) regulates both events. Thus, ORNs utilize diverse strategies and complex regulatory networks to coordinate their physiology and connectivity.

Indexed as

TranscriptomeAnimalsAxonsDrosophila melanogasterDrosophila ProteinsForkhead Transcription FactorsHomeodomain ProteinsNerve Tissue ProteinsOlfactory Receptor NeuronsPOU Domain FactorsReceptors, OdorantSingle-Cell AnalysisSmellacj6 protein, DrosophilaDrosophila Proteinsfkh protein, DrosophilaForkhead Transcription FactorsHomeodomain ProteinsNerve Tissue ProteinsPOU Domain FactorsReceptors, Odorantunpg protein, Drosophilaaxon targetingDrosophilaglomerulineuronal typeolfactory receptorolfactory receptor neuronsolfactory systemsingle-cell RNA-seq

Identifiers

PMID32059767
PMCPMC7231479
OpenAlexW3005974692

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.