ArticlePloS one2020
Super resolution measurement of collagen fibers in biological samples: Validation of a commercial solution for multiphoton microscopy.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Evaluating the applicability of computational super-resolution techniques for harmonic generation microscopy.Scientific reports · 2026Article
- MuscleX-DI: an integrated data analysis package for X-ray scanning diffraction imaging experiments.Journal of synchrotron radiation · 2026Article
- Exploring Multi-Organ Crosstalk via the TissUse HUMIMIC Chip System: Lessons Learnt So Far.Biotechnology and bioengineering · 2025Review
- Geometric characteristics of stromal collagen fibres in breast cancer using differential interference contrast microscopy.Journal of microscopy · 2025Article
- Profiling of collagen and extracellular matrix deposition from cell culture using in vitro ExtraCellular matrix mass spectrometry imaging (ivECM-MSI).Matrix biology plus · 2024Article
- Molecular histopathology of matrix proteins through autofluorescence super-resolution microscopy.Scientific reports · 2024Article
- Viewing life without labels under optical microscopes.Communications biology · 2023Review
- CRISPR/Cas9-Mediated Allele-Specific Disruption of a DominantInternational journal of molecular sciences · 2022Article
- Association of Tumor-Associated Collagen Signature With Prognosis and Adjuvant Chemotherapy Benefits in Patients With Gastric Cancer.JAMA network open · 2021Article
- Review
- Second harmonic generation imaging of collagen scaffolds within the alveolar ducts of healthy and emphysematous mouse lungs.Histochemistry and cell biology · 2021Article
- Simplified computational model for generating biological networks.RSC advances · 2020Article
- Epithelial-interleukin-1 inhibits collagen formation by airway fibroblasts: Implications for asthma.Scientific reports · 2020Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Multiphoton microscopy is a powerful, non-invasive technique to image biological specimens. One current limitation of multiphoton microscopy is resolution as many of the biological molecules and structures investigated by research groups are similar in size or smaller than the diffraction limit. To date, the combination of multiphoton and super-resolution imaging has proved technically challenging for biology focused laboratories to implement. Here we validate that the commercial super-resolution Airyscan detector from ZEISS, which is based on image scanning microscopy, can be integrated under warranty with a pulsed multi-photon laser to enable multiphoton microscopy with super-resolution. We demonstrate its biological application in two different imaging modalities, second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), to measure the fibre thicknesses of collagen and elastin molecules surpassing the diffraction limit by a factor of 1.7±0.3x and 1.4±0.3x respectively, in human heart and lung tissues, and 3-dimensional in vitro models. We show that enhanced resolution and signal-to-noise of SHG using the Airyscan compared to traditional GaAs detectors allows for automated and precise measurement of collagen fibres using texture analysis in biological tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.