Evidence map›Paper›PMID 32056953›Full record

ArticleBiosensors & bioelectronics2020

Cellular sensing platform with enhanced sensitivity based on optogenetic modulation of cell homeostasis.

Mihaela Gheorghiu, Luciana Stănică, Miruna G Ghinia Tegla, Cristina Polonschii, Dumitru Bratu, Octavian Popescu, Tudor Badea, Eugen Gheorghiu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Mihaela GheorghiuInternational Centre of Biodynamics, Intr. Portocalelor 1 B, 060101, Bucharest, Romania; Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest, 050095, Romania. Electronic address: mgheorghiu@biodyn.ro.
Luciana StănicăInternational Centre of Biodynamics, Intr. Portocalelor 1 B, 060101, Bucharest, Romania; Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest, 050095, Romania.
Miruna G Ghinia TeglaInstitute for Interdisciplinary Research in Bio-Nano-Sciences, Molecular Biology Center, Babes-Bolyai-University, 400084, Cluj-Napoca, Romania; Retinal Circuit Development & Genetics Unit N-NRL/NEI/NIH 6 Center Drive Bethesda, 20892, Maryland, United States.
Cristina PolonschiiInternational Centre of Biodynamics, Intr. Portocalelor 1 B, 060101, Bucharest, Romania. Electronic address: cpolonschii@biodyn.ro.
Dumitru BratuInternational Centre of Biodynamics, Intr. Portocalelor 1 B, 060101, Bucharest, Romania. Electronic address: dbratu@biodyn.ro.
Octavian PopescuInstitute for Interdisciplinary Research in Bio-Nano-Sciences, Molecular Biology Center, Babes-Bolyai-University, 400084, Cluj-Napoca, Romania; Institute of Biology, Romanian Academy, 296 Splaiul Independentei, 060031, Bucharest, Romania.
Tudor BadeaRetinal Circuit Development & Genetics Unit N-NRL/NEI/NIH 6 Center Drive Bethesda, 20892, Maryland, United States. Electronic address: tudor.badea@nih.gov.
Eugen GheorghiuInternational Centre of Biodynamics, Intr. Portocalelor 1 B, 060101, Bucharest, Romania; Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest, 050095, Romania. Electronic address: egheorghiu@biodyn.ro.
International Centre of Biodynamics · ROBabeș-Bolyai University · ROUniversity of Bucharest · RO

Funding

Retinal Circuit Development & GeneticsZIAEY000504 · NEI · NATIONAL EYE INSTITUTE · PI BADEA, TUDOR · 2011 to 2021
$15.6M
Intramural NIH HHS ZIA EY000504
6 · The paper itself

Abstract

We demonstrate a new biosensing concept with impact on the development of rapid, point of need cell based sensing with boosted sensitivity and wide relevance for bioanalysis. It involves optogenetic stimulation of cells stably transfected to express light sensitive protein channels for optical control of membrane potential and of ion homeostasis. Time-lapse impedance measurements are used to reveal cell dynamics changes encompassing cellular responses to bioactive stimuli and optically induced homeostasis disturbances. We prove that light driven perturbations of cell membrane potential induce homeostatic reactions and modulate transduction mechanisms that amplify cellular response to bioactive compounds. This allows cell based biosensors to respond more rapidly and sensitively to low concentrations of bioactive/toxic analytes: statistically relevant impedance changes are recorded in less than 30 min, in comparison with >8 h in the best alternative reported tests for the same low concentration (e.g. a concentration of 25 μM CdCl

Indexed as

Biosensing TechniquesHomeostasisHumansIonsOptogeneticsOuabainIonsOuabainCellular based biosensing platforms (CBS)Cellular dynamicsLight driven homeostasisOptogeneticsReal-time label-free non-invasive analysis

Identifiers

PMID32056953
PMCPMC7685521
OpenAlexW2997187762

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.