Evidence map›Paper›PMID 32056730›Full record

ArticleBiosensors & bioelectronics2020

Label-free enrichment of fate-biased human neural stem and progenitor cells.

Tayloria N G Adams, Alan Y L Jiang, Nicolo S Mendoza, Clarissa C Ro, Do-Hyun Lee, Abraham P Lee, Lisa A Flanagan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Tayloria N G AdamsDepartment of Chemical and Biomolecular Engineering, University of California, Irvine, Irvine, CA, 92697-2580, USA; Department of Neurology, University of California, Irvine, Irvine, CA, 92697-6750, USA; Sue & Bill Gross Stem Cell Research Center, University of California, Irvine, CA, 92697-1705, USA. Electronic address: tayloria@uci.edu.
Alan Y L JiangDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, 92697-2627, USA; Department of Neurology, University of California, Irvine, Irvine, CA, 92697-6750, USA; Sue & Bill Gross Stem Cell Research Center, University of California, Irvine, CA, 92697-1705, USA.
Nicolo S MendozaDepartment of Neurology, University of California, Irvine, Irvine, CA, 92697-6750, USA; Sue & Bill Gross Stem Cell Research Center, University of California, Irvine, CA, 92697-1705, USA.
Clarissa C RoDepartment of Neurology, University of California, Irvine, Irvine, CA, 92697-6750, USA; Sue & Bill Gross Stem Cell Research Center, University of California, Irvine, CA, 92697-1705, USA.
Do-Hyun LeeDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, 92697-2627, USA.
Abraham P LeeDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, 92697-2627, USA.
Lisa A FlanaganDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, 92697-2627, USA; Department of Neurology, University of California, Irvine, Irvine, CA, 92697-6750, USA; Sue & Bill Gross Stem Cell Research Center, University of California, Irvine, CA, 92697-1705, USA; Department of Anatomy & Neurobiology, University of California, Irvine, Irvine, CA, 92697-4291, USA. Electronic address: lisa.flanagan@uci.edu.

Funding

Institute for Clinical and Translational ScienceUL1TR000153 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI COOPER, DAN M · 2012 to 2015
$12.1M
NCATS NIH HHS UL1 TR000153
6 · The paper itself

Abstract

Human neural stem and progenitor cells (hNSPCs) have therapeutic potential to treat neural diseases and injuries since they provide neuroprotection and differentiate into astrocytes, neurons, and oligodendrocytes. However, cultures of hNSPCs are heterogeneous, containing cells linked to distinct differentiated cell fates. HNSPCs that differentiate into astrocytes are of interest for specific neurological diseases, creating a need for approaches that can detect and isolate these cells. Astrocyte-biased hNSPCs differ from other cell types in electrophysiological properties, namely membrane capacitance, and we hypothesized that this could be used to enrich these cells using dielectrophoresis (DEP). We implemented a two-step DEP sorting scheme, consisting of analysis to define the optimal sorting frequency followed by separation of cells at that frequency, to test whether astrocyte-biased cells could be separated from the other cell types present in hNSPC cultures. We developed a novel device that increased sorting reproducibility and provided both enriched and depleted cell populations in a single sort. Astrocyte-biased cells were successfully enriched from hNSPC cultures by DEP sorting, making this the first study to use electrophysiological properties for label-free enrichment of human astrocyte-biased cells. Enriched astrocyte-biased human cells enable future experiments to determine the specific properties of these important cells and test their therapeutic efficacy in animal models of neurological diseases.

Indexed as

Lab-On-A-Chip DevicesAstrocytesBiosensing TechniquesCell LineCell SeparationElectric CapacitanceEquipment DesignHumansNeural Stem CellsNeuronsOligodendrogliaAstrocyteCell sortingDielectrophoresisMembrane capacitanceMicrofluidicNeural stem and progenitor cell

Identifiers

PMID32056730
PMCPMC8860404

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.