ArticleNature communications2020
Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 82 citations in OpenAlex.
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- Life beyond 6000 genes: from sequence to synthesis in a post-genomics era.FEMS yeast research · 2026Review
- EASY-C: Extraction and Analysis of Small Yeast Chromosomes-A rapid and universal platform for recovering artificial mini-chromosomes from synthetic Sc2.0 yeast and large plasmids fromSynthetic biology (Oxford, England) · 2026Article
- Advances and challenges in betulinic acid therapeutics and delivery systems for breast cancer prevention and treatment.RSC advances · 2025Review
- Iterative SCRaMbLE for engineering synthetic genome modules and chromosomes.Nature communications · 2025Article
- New Insight into Microbial Exploitation to Produce Bioactive Molecules from Agrifood and By-Products' Fermentation.Foods (Basel, Switzerland) · 2025Review
- Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome.Nature communications · 2025Article
- Identification of a dual functional betulinic acid analog for the treatment of osteoarthritis by phenotypic screening.Science China. Life sciences · 2025Article
- Synthetic Genomics: Repurposing Biological Systems for Applications in Engineering Biology.ACS synthetic biology · 2024Review
- An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts.ACS synthetic biology · 2024Article
- Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts.Nature communications · 2024Article
- Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast.Nature communications · 2024Article
- Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae.Nature communications · 2024Article
- Article
- A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid.Nature communications · 2023Article
- A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering.Communications biology · 2023Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering.Research square · 2023Article
- Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis inSynthetic and systems biotechnology · 2023Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Synthetic biology, genome engineering and directed evolution offer innumerable tools to expedite engineering of strains for optimising biosynthetic pathways. One of the most radical is SCRaMbLE, a system of inducible in vivo deletion and rearrangement of synthetic yeast chromosomes, diversifying the genotype of millions of Saccharomyces cerevisiae cells in hours. SCRaMbLE can yield strains with improved biosynthetic phenotypes but is limited by screening capabilities. To address this bottleneck, we combine automated sample preparation, an ultra-fast 84-second LC-MS method, and barcoded nanopore sequencing to rapidly isolate and characterise the best performing strains. Here, we use SCRaMbLE to optimise yeast strains engineered to produce the triterpenoid betulinic acid. Our semi-automated workflow screens 1,000 colonies, identifying and sequencing 12 strains with between 2- to 7-fold improvement in betulinic acid titre. The broad applicability of this workflow to rapidly isolate improved strains from a variant library makes this a valuable tool for biotechnology.
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