Evidence map›Paper›PMID 32054545›Full record

ArticleEpidemiology and infection2020

Phenotypic and molecular characterisation of a novel species,

Xiaoli Yu, Hui Zheng, Fang Zhou, Peng Hu, Hualin Wang, Na Li, Juncai He, Peidong Wang, Lu Zhang, Hongsheng Men and 2 more

Open access · goldAbstract read
In one paragraph

Article in Epidemiology and infection, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Xiaoli YuSchool of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.ORCID 0000-0002-6604-6429
Hui ZhengSchool of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
Fang ZhouSchool of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
Peng HuSchool of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
Hualin WangSchool of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
Na LiSchool of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
Juncai HeSchool of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
Peidong WangSchool of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
Lu ZhangSchool of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
Hongsheng MenDepartment of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA.
Jie XiangWuhan Medical Treatment Center, Wuhan, China.
Shulin ZhangDepartment of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Wuhan Polytechnic University · CNJinyintan Hospital · CNShanghai Jiao Tong University · CNUniversity of Missouri · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A new fast-growing mycobacterium, designated strain QGD101T, was isolated from the sputum of an 84-year-old man suspected of tuberculosis in Wuhan Medical Treatment Center, Hubei, China. This strain was a gram-staining-negative, aerobic, non-spore-forming and catalase-positive bacterium, which was further identified as the NTM by PNB and TCH tests. The moxifloxacin and levofloxacin exhibited strong suppressing function against QGD101T with MIC values of 0.06 and 0.125 µg/ml after drug susceptibility testing of six main antimicrobial agents on mycobacteria. Based on the sequence analysis of 16S rRNA, rpoB, hsp65 and 16S-23S rRNA internal transcribed spacer, the strain QGD101T could not be identified to a species level. Mycobacterium moriokaense ATCC43059T that shared the highest 16S rRNA gene sequence similarity (98%) with strain QGD101T was actually different in genomes average nucleotide identity (78.74%). In addition, the major cellular fatty acids of QGD101T were determined as C18:1ω9c, C16:0 and C18:2ω6c. The DNA G + C content was 64.9% measured by high performance liquid chromatography. Therefore, the phenotypic and genotypic characterisation of this strain led us to the conclusion that it represents a novel species of mycobacteria, for which the name Mycobacterium hubeiense sp. nov. (type strain QGD101T = CCTCCAA 2017003T = KCTC39927T) was proposed. Thus, the results of this study are very significant for the clinical diagnosis of tuberculosis and future personalised medicine.

Indexed as

Aged, 80 and overAntitubercular AgentsBacterial ProteinsBase CompositionChaperonin 60ChinaCluster AnalysisCytosolDNA, BacterialDNA-Directed RNA PolymerasesDNA, RibosomalDNA, Ribosomal SpacerFatty AcidsHumansLevofloxacinMaleAntitubercular AgentsBacterial ProteinsChaperonin 60DNA, BacterialDNA-Directed RNA PolymerasesDNA, RibosomalDNA, Ribosomal SpacerFatty Acidsheat-shock protein 65, MycobacteriumLevofloxacinMoxifloxacinRNA polymerase beta subunitRNA, Ribosomal, 16S16S rRNAgenomic analysisnontuberculous mycobacteriaphenotyperpoB

Identifiers

PMID32054545
PMCPMC7078510
OpenAlexW3006620876

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.